Song Linhao, Shrivastava Neeraj, Gai Yunpeng, Li Dong, Cai Weiming, Shen Yingyue, Lin Fu-Cheng, Liu Jingyu, Wang Hongkai
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Shanxi Key Laboratory of Edible Fungi for Loess Plateau, College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi, China.
Front Microbiol. 2023 Jan 10;13:1038034. doi: 10.3389/fmicb.2022.1038034. eCollection 2022.
The , is well known highly prized medicinal mushroom with great demand in food and pharmaceutical industry. Due to its economic value and therapeutic uses, natural sources of wild are over-exploited and reducing continuously. Therefore, commercial cultivation in controlled environment is an utmost requirement to fulfill the consumer's demand. Due to the lack of knowledge on fruiting body (synnemata) development and regulation, commercial cultivation is currently in a difficult situation. In the growth cycle of macrofungi, such as mushrooms, light is the main factor affecting growth and development, but so far, specific effects of light on the growth and development of is unknown. In this study, we identified a blue light receptor white-collar-1 () gene homologue with well-defined functions in morphological development in based on gene knockout technology and transcriptomic analysis. It was found that the gene significantly affected hyphal growth and fruiting body development. This study confirms that acts as an upstream regulatory gene that regulates genes associated with fruiting body formation, pigment-forming genes, and related genes for enzyme synthesis. Transcriptome data analysis also found that affects many important metabolic pathways of , i.e., amino acid metabolism and fatty acid metabolism. The above findings will not only provide a comprehensive understanding about the molecular mechanism of light regulation in , but also provide new insights for future breeding program and improving this functional food production.
该[物种名称]是一种著名的、备受珍视的药用蘑菇，在食品和制药行业有很大需求。由于其经济价值和治疗用途，野生[物种名称]的天然来源被过度开发且持续减少。因此，在可控环境中进行商业栽培是满足消费者需求的迫切要求。由于缺乏关于子实体（束丝）发育和调控的知识，目前商业栽培面临困境。在大型真菌如蘑菇的生长周期中，光是影响生长发育的主要因素，但到目前为止，光对[物种名称]生长发育的具体影响尚不清楚。在本研究中，我们基于基因敲除技术和转录组分析，鉴定出一个在[物种名称]形态发育中具有明确功能的蓝光受体白领-1（[基因名称]）基因同源物。发现[基因名称]基因显著影响菌丝生长和子实体发育。本研究证实[基因名称]作为上游调控基因，调控与子实体形成相关的基因、色素形成基因以及酶合成相关基因。转录组数据分析还发现[基因名称]影响[物种名称]的许多重要代谢途径，即氨基酸代谢和脂肪酸代谢。上述发现不仅将提供对[物种名称]光调控分子机制的全面理解，还将为未来的育种计划和改进这种功能性食品生产提供新的见解。
