Mulet Magdalena, Gomila Margarita, Lalucat Jorge, Bosch Rafael, Rossello-Mora Ramon, García-Valdés Elena
Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain.
Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain; Grup Microbiologia del Medi Ambient, Institut Mediterrani d'Estudis Avançats (IMEDEA, CSIC-UIB), Campus UIB, 07122 Palma de Mallorca, Spain.
Syst Appl Microbiol. 2023 Apr;46(2):126400. doi: 10.1016/j.syapm.2023.126400. Epub 2023 Jan 23.
Strains 19SMN4 and ST27MN3 were isolated from marine sediments after enrichment with 2-methylnaphthalene and were classified as Pseudomonas stutzeri genomovar 4. Four other strains, BG 2, HT20, HT24, and A7, were isolated from sulphide-oxidizing bioreactors or activated sludge affiliated with the same clade in the 16S rRNA phylogenetic tree. P. stutzeri has been recently reclassified as a new genus, Stutzerimonas, and a preliminary analysis indicated that the strains in this study were distinct from any classified Stutzerimonas and are considered representatives of phylogenomic species 4 (pgs4). Strains 19SMN4 and ST27MN3 were extensively characterized with phenotypic, chemotaxonomic, genomic and phylogenomic data. Strain 19SMN4 had a well-characterized naphthalene degradative plasmid that has been compared with other plasmids, while in strain ST27MN3, the naphthalene degradative genes were detected in the chromosome sequence. Phylogenomic analysis of the core gene sequences showed that strains 19SMN4 and ST27MN3 shared 3,995 genes and were closely related to members of the species "Stutzerimonas songnenensis" and Stutzerimonas perfectomarina, as well as to the Stutzerimonas phylogenomic species, pgs9, pgs16 and pgs24. The aggregate average nucleotide identity (ANI) indicated that strains 19SMN4 and ST27MN3 belonged to the same genomic species, whereas the genomic indices with their closest-related type strains were below the accepted species threshold (95 %). We therefore conclude that strains 19SMN4 and ST27MN3 represent a novel species of Stutzerimonas, for which the name Stutzerimonas decontaminans is proposed; the type strain is 19SMN4 (=CCUG44593 = DSM6084 = LMG18521).
菌株19SMN4和ST27MN3是在以2-甲基萘富集后从海洋沉积物中分离得到的，被归类为斯氏假单胞菌基因组变种4。另外四个菌株BG 2、HT20、HT24和A7是从16S rRNA系统发育树中属于同一进化枝的硫化物氧化生物反应器或活性污泥中分离得到的。斯氏假单胞菌最近已被重新分类为一个新属，即斯氏单胞菌属，初步分析表明本研究中的菌株与任何已分类的斯氏单胞菌不同，被认为是系统发育种4（pgs4）的代表。菌株19SMN4和ST27MN3通过表型、化学分类、基因组和系统发育基因组数据进行了广泛的表征。菌株19SMN4有一个特征明确的萘降解质粒，该质粒已与其他质粒进行了比较，而在菌株ST27MN3中，萘降解基因在染色体序列中被检测到。核心基因序列的系统发育基因组分析表明，菌株19SMN4和ST27MN3共有3995个基因，与“松嫩斯氏单胞菌”和完美斯氏单胞菌的成员以及斯氏单胞菌系统发育种pgs9、pgs16和pgs24密切相关。总体平均核苷酸同一性（ANI）表明菌株19SMN4和ST27MN3属于同一基因组种，而与它们关系最密切的模式菌株的基因组指数低于公认的种阈值（95%）。因此，我们得出结论，菌株19SMN4和ST27MN3代表斯氏单胞菌属的一个新种，为此提出名称为去污斯氏单胞菌；模式菌株为19SMN4（=CCUG44593 = DSM6084 = LMG18521）。
