Moresi Naomi G, Geck Renee C, Skophammer Ryan, Godin Dennis, Taylor M Bryce, Dunham Maitreya J
Department of Genome Sciences, University of Washington, Seattle, WA 98195.
Westridge School, Pasadena, CA 91105.
bioRxiv. 2023 Jan 20:2023.01.17.524437. doi: 10.1101/2023.01.17.524437.
yEvo is a curriculum for high school students centered around evolution experiments in . To adapt the curriculum for remote instruction, we created a new protocol to evolve non-GMO yeast in the presence of caffeine. Evolved strains had increased caffeine tolerance and distinct colony morphologies. Many possessed copy number variations, transposon insertions, and mutations affecting genes with known relationships to caffeine and TOR signaling - which is inhibited by caffeine - and in other genes not previously connected with caffeine. This demonstrates that our accessible, at-home protocol is sufficient to permit novel insights into caffeine tolerance.
yEvo是一门面向高中生的课程，围绕[具体地点]的进化实验展开。为了使该课程适用于远程教学，我们创建了一种新方案，让非转基因酵母在咖啡因存在的情况下进化。进化后的菌株对咖啡因的耐受性增强，菌落形态也有所不同。许多菌株存在拷贝数变异、转座子插入以及影响与咖啡因和TOR信号通路（该信号通路会被咖啡因抑制）具有已知关系的基因的突变，还有一些突变发生在之前与咖啡因没有关联的其他基因中。这表明我们这种便于在家操作的方案足以让我们对咖啡因耐受性有新的认识。
