Mogharehabed Ahmad, Torabinia Nakisa, Sharifi Darani Sare, Afshari Zohreh, Yaghini Jaber
Department of Periodontics, Dental Implants Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Oral and Maxillofacial Pathology, Dental Materials Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
J Adv Periodontol Implant Dent. 2022 Aug 6;14(2):89-96. doi: 10.34172/japid.2022.011. eCollection 2022.
Recently, the use of leukocyte- and platelet-rich fibrin (L-PRF) has been recommended due to the presence of various growth factors to increase the success of free gingival grafts (FGG). This study evaluated the effect of using L-PRF in the healing of FGG in rabbits. Twenty rabbits were randomly divided into two groups. In each group, FGG was performed in two separate sites with or without L-PRF. One of these groups was sacrificed on the 7th day and the other on the 28th day and analyzed in terms of clinical indices, including wound healing, gingi-val thickness (GT), and keratinized tissue width (KTW). Then histologic sections were obtained and stained for type and degree of inflammation and rate of vascular formation analysis. SPSS 22 was used for statistical analysis. The extent of changes in GT, KTW, wound healing index, and vascular formation between the test and control groups was not statistically significant. The difference in the type of inflammation was significant only between the -7day and -28day control groups (P=0.003). The degree of inflammation between the -7day test group and the -28day control group, as well as the -7day and -28day control groups, were statistically significant (P=0.011 and P=0.002, respectively). Using L-PRF with FGG could improve FGG healing compared to using FGG alone, but the results were not statistically significant.
最近，由于富含白细胞和血小板的纤维蛋白（L-PRF）中存在多种生长因子，可提高游离龈瓣移植术（FGG）的成功率，因此有人推荐使用该物质。本研究评估了L-PRF在兔FGG愈合过程中的作用。将20只兔随机分为两组。每组在两个不同部位进行FGG手术，一组使用L-PRF，另一组不使用。其中一组在第7天处死，另一组在第28天处死，并根据临床指标进行分析，包括伤口愈合、牙龈厚度（GT）和角化组织宽度（KTW）。然后获取组织学切片，进行炎症类型和程度以及血管形成率分析的染色。使用SPSS 22进行统计分析。试验组和对照组之间GT、KTW、伤口愈合指数和血管形成的变化程度无统计学意义。仅在第7天和第28天的对照组之间炎症类型存在显著差异（P = 0.003）。第7天试验组与第28天对照组之间以及第7天和第28天对照组之间的炎症程度具有统计学意义（分别为P = 0.011和P = 0.002）。与单独使用FGG相比，FGG联合使用L-PRF可改善FGG愈合，但结果无统计学意义。
