Mahmoudi Shaida, Chaichi Mohamad J, Shamsipur Mojtaba, Nazari O Leila, Samadi Maybodi Abdol R
Department of Analytical Chemistry, University of Mazandaran, Babolsar, Iran.
Department of Analytical Chemistry, University of Razi, Kermanshah, Iran.
Heliyon. 2023 Jan 7;9(2):e12866. doi: 10.1016/j.heliyon.2023.e12866. eCollection 2023 Feb.
In this article; the bimetal metal-organic framework Zn/Mg (Zn/Mg MOF) is synthesized. Then Zn/Mg MOF bimetal was combined with FeO and FeO@SiO, and composites of FeO@ SiO/MOF/Dextrin, FeO@SiO/MOF, FeO@MOF/Dextrin and FeO@MOF made. The peroxidase-like activity of these compounds was investigated and compared by calorimetric Resazurin (Rz) and O-phenylenediamine (OPD); (Rz-HO, OPD-HO) and fluorimetric Rz and terephtalic acid (TA); (Rz-HO, TA-HO). The FeO@ MOF/Dextrin composite has the highest peroxidase-like activity. The effect factors (amount of pH (6), the values of TA (1.37 mM), HO (0.025 mM), reaction time (8.15 min), and amount of Composite (116.67 mg)) to increase the catalytic activity of FeO@ MOF/Dextrin measured by chemometrics method. The most suitable linear range of the calibration curve by the TA-HO -Composite fluorimetric method is 1-600 μg L, and the detection limit is 2.27 μg L. The relative standard deviation (RSD%) for measuring concentration atropine 1 μg L (n = 6) is 1.18%. Finally, from this system for measuring atropine extracted by the Liquid-liquid extraction (LLE) method in two types of plants, D. Innoxia north and west and D. stramonium north and west of Iran (118.25 μg L, 79.80 μg L) and (18.477 μg L, 9.27 μg L) used, respectively.
在本文中，合成了双金属金属有机框架Zn/Mg（Zn/Mg MOF）。然后将Zn/Mg MOF双金属与FeO和FeO@SiO相结合，制备了FeO@SiO/MOF/糊精、FeO@SiO/MOF、FeO@MOF/糊精和FeO@MOF复合材料。通过比色法刃天青（Rz）和邻苯二胺（OPD）；（Rz-H₂O₂，OPD-H₂O₂）以及荧光法Rz和对苯二甲酸（TA）；（Rz-H₂O₂，TA-H₂O₂）研究并比较了这些化合物的过氧化物酶样活性。FeO@MOF/糊精复合材料具有最高的过氧化物酶样活性。通过化学计量学方法测定了提高FeO@MOF/糊精催化活性的影响因素（pH值为6、TA值为1.37 mM、H₂O₂为0.025 mM、反应时间为8.15分钟以及复合材料用量为116.67 mg）。TA-H₂O₂-复合材料荧光法校准曲线的最适线性范围为1-600 μg L，检测限为2.27 μg L。测定1 μg L阿托品浓度时的相对标准偏差（RSD%）（n = 6）为1.18%。最后，利用该体系通过液液萃取（LLE）法测定了伊朗西北部的无刺曼陀罗和曼陀罗两种植物中提取的阿托品，分别为（118.25 μg L，79.80 μg L）和（18.477 μg L，9.27 μg L）。
