Sotthibandhu Dennapa Saeloh, Indoung Saowakon, Niwasawat Husna, Chaiboon Jiradchaya, Sungsorn Nattakan, Longji Nu-Issana, Polya Kittipol, Noosak Chayanee, Schwarz Stefan, Soimala Tanawan
Faculty of Medical Technology, Prince of Songkla University, Songkhla 90110, Thailand.
Faculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, Thailand.
Vet World. 2022 Dec;15(12):2922-2928. doi: 10.14202/vetworld.2022.2922-2928. Epub 2022 Dec 24.
fighting bulls have a high risk of eye injuries, and opportunistic conjunctival bacterial flora may cause subsequent eye diseases. There is little information about the ocular health care of fighting bulls in Thailand. Thus, this study aimed to estimate the prevalence of spp. from the eyes of fighting bulls and investigate their antimicrobial susceptibility.
The samples were collected from the right conjunctival sacs of 105 fighting bulls. Biochemical tests and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry were used to identify bacteria to genus and species levels. Antimicrobial susceptibility was tested by agar disk diffusion.
spp. (36.84%, 56/152) were the most detected bacteria. The most prevalent spp. was (37.50%, 21/56). The susceptibility test revealed that all isolates were susceptible to sulfamethoxazole/trimethoprim (56/56, 100%) and most were susceptible to chloramphenicol and gentamicin (54/56, 96.43%). The highest resistance rates were seen for tetracycline and doxycycline (23.21%, 13/56) followed by erythromycin (19.64%, 11/56). In addition, isolates were evaluated for their ability to produce biofilms by a quantitative biofilm production assay. A total of 21 isolates exhibited biofilm production, independent of their antimicrobial susceptibility. Three multidrug-resistant isolates were found, including two isolates and a single isolate.
As antimicrobial resistant bacteria were detected on the eye surface, veterinarians should always conduct antimicrobial susceptibility testing before using antimicrobial agents. The results from this study will help to improve the standard of eye treatment for fighting bulls in Thailand.
斗牛眼部受伤风险高，结膜机会性细菌菌群可能引发后续眼部疾病。泰国关于斗牛眼部保健的信息较少。因此，本研究旨在评估斗牛眼部 spp. 的流行情况并调查其抗菌药物敏感性。
从105头斗牛的右侧结膜囊中采集样本。采用生化试验和基质辅助激光解吸/电离飞行时间质谱法将细菌鉴定到属和种水平。通过琼脂纸片扩散法检测抗菌药物敏感性。
spp.（36.84%，56/152）是检测到的最常见细菌。最常见的 spp. 是 （37.50%，21/56）。药敏试验显示，所有分离株对磺胺甲恶唑/甲氧苄啶均敏感（56/56，100%），大多数对氯霉素和庆大霉素敏感（54/56，96.43%）。四环素和多西环素的耐药率最高（23.21%，13/56），其次是红霉素（19.64%，11/56）。此外，通过定量生物膜生成试验评估分离株产生生物膜的能力。共有21株分离株表现出生物膜生成能力，与它们的抗菌药物敏感性无关。发现3株多重耐药分离株，包括2株 分离株和1株 分离株。
由于在眼表检测到了抗菌药物耐药菌，兽医在使用抗菌药物前应始终进行抗菌药物敏感性检测。本研究结果将有助于提高泰国斗牛眼部治疗的标准。
