腹腔注射落花生作为肾脏保护剂的药物开发。

Pharmaceutical Development of Intraperitoneal Arachis hypogaea as a Renal Protective Agent.

机构信息

Department of Pharmacy, Abdul Wali Khan University Mardan, 23200 Mardan, Pakistan.

Department of Clinical Laboratory, College of Applied Medical Science, Taif University, 21944 Taif, Saudi Arabia.

出版信息

Front Biosci (Landmark Ed). 2023 Jan 17;28(1):14. doi: 10.31083/j.fbl2801014.

DOI:10.31083/j.fbl2801014

PMID:36722262

Abstract

BACKGROUND

Kidneys are among the vital organs of the human body; therefore, damage from any exogenous/endogenous agent may put human life at risk. Arachis hypogaea (AH) contains different free radical scavenging flavonoids, stilbenes, and tannins. This research aimed to elucidate the possible nephroprotective mechanism of AH methanolic crude extract (AHcr) and n-hexane oil fraction (AHO) against gentamycin-induced nephrotoxicity.

METHODS

After the extraction of the crude oil of the plant, they were tested against a Gentamycin (GM)-treated group of Swiss Albino mice for their nephroprotective action. Animals were divided into six (6) equal groups with five (5) animals in each group. These groups were: control group (0.5 mL normal saline via intraperitoneal -i.p), gentamycin group (gentamycin 100 mg/kg i.p), Silymarin + gentamycin group (Silymarin 50 mg/kg and gentamycin 100 mg/kg i.p), plant extract (AHcr1) and gentamycin group (AHcr1 250 mg/kg and gentamycin 100 mg/kg i.p), AHcr2 + gentamycin group (AHcr2; 500 mg/kg and gentamycin 100 mg/kg i.p) and the hexane oil fraction (AHO) + gentamycin (AHO 1 mL/kg and GM 100 mg/kg i.p). After completion of doses, animals were sacrificed for the collection of blood to further investigate biochemical changes and histopathological changes in kidney tissues.

RESULTS

Serum creatinine, urea, and blood urea nitrogen significantly increased ( < 0.001) in the gentamycin-treated group as compared to the control group. The elevated level of serum creatinine, urea, and blood urea nitrogen was decreased significantly ( < 0.001) in groups treated with AHcr and AHO compared to the gentamycin group. Similarly, the histopathological study of kidney tissues from the gentamycin group showed tubular necrosis, vacuolation, and fibrosis.

CONCLUSIONS

The effect of crude extract and hexane soluble fraction of AH caused a significant reversal of gentamycin-induced nephrotoxicity.

摘要

背景

肾脏是人体的重要器官之一；因此，任何外源性/内源性物质的损伤都可能危及人类生命。落花生（AH）含有不同的自由基清除黄酮类、芪类和单宁。本研究旨在阐明 AH 甲醇粗提物（AHcr）和正己烷油馏分（AHO）对庆大霉素诱导的肾毒性的可能肾保护机制。

方法

在提取植物粗油后，对瑞士白化病小鼠进行了庆大霉素（GM）处理组的肾保护作用测试。动物分为六（6）组，每组五（5）只。这些组是：对照组（0.5mL 生理盐水经腹腔内给药）-i.p)、庆大霉素组（庆大霉素 100mg/kg i.p)、水飞蓟素+庆大霉素组（水飞蓟素 50mg/kg 和庆大霉素 100mg/kg i.p)、植物提取物（AHcr1)和庆大霉素组（AHcr1 250mg/kg 和庆大霉素 100mg/kg i.p)、AHcr2+庆大霉素组（AHcr2;500mg/kg 和庆大霉素 100mg/kg i.p)和正己烷油馏分（AHO)和庆大霉素（AHO 1mL/kg 和 GM 100mg/kg i.p)。剂量完成后，处死动物以收集血液，进一步研究肾脏组织的生化变化和组织病理学变化。