Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway.

OBJECTIVE

The bacterial cellulose membrane (BCM) has been widely studied and applied as a new biomaterial for wound healing, but causes pain with frequent dressing changes. Local application of bone marrow mesenchymal stem cells (BMSCs) requires a niche. Furthermore, the effect and mechanism of the BCM combined with BMSCs have not been reported.

METHODS

Morphological and chemical identifications of BCMs were investigated by porosity analyses, scanning electron microscopy, and Fourier-transform infrared spectroscopy. Biological wound dressings (BWDs) were prepared by the BCM in combination with BMSCs. The biological effects of BWDs on human dermal fibroblast (HDF) and VEGF-A in human vascular endothelial cells (HuVECs) were detected , and the effect of BWDs on acute wounds in mice was detected . Collagen and angiogenesis were evaluated through hematoxylin-eosin staining and Masson staining. The expressions of and and the activation of the Notch signaling pathway and were detected by quantitative reverse-transcriptase polymerase chain reaction.

RESULTS

The BCM had a nanoscale structure and provided a partial niche for the survival and proliferation of BMSCs. BWDs were successfully prepared and regulated the biological behaviors of wound healing-related cells and upregulated the expressions of in HDF and in HuVECs. BWDs promoted wound healing by increasing collagen type I synthesis and angiogenesis in acute wounds in mice.

CONCLUSIONS

BWDs prepared by the combination of nanomaterial BCMs and BMSCs facilitated acute wound healing, which may be regulated by activating the Notch signaling pathway.