Wang Xiaoyang, Zhao Jie, Wang Xiaochuan, Zhang Jingjuan, Wang Yi, Wang Xinyue, Jia Shanshan, Shi Nian, Lu Meiqi, Su Hongxia, Zhang Jixun, Jiang Duyin
Department of Plastic and Burns Surgery, The Second Hospital of Shandong University, Jinan, China.
Emergency Medicine Center, The Second Hospital of Shandong University, Jinan, China.
Front Surg. 2023 Jan 16;9:1027067. doi: 10.3389/fsurg.2022.1027067. eCollection 2022.
The bacterial cellulose membrane (BCM) has been widely studied and applied as a new biomaterial for wound healing, but causes pain with frequent dressing changes. Local application of bone marrow mesenchymal stem cells (BMSCs) requires a niche. Furthermore, the effect and mechanism of the BCM combined with BMSCs have not been reported.
Morphological and chemical identifications of BCMs were investigated by porosity analyses, scanning electron microscopy, and Fourier-transform infrared spectroscopy. Biological wound dressings (BWDs) were prepared by the BCM in combination with BMSCs. The biological effects of BWDs on human dermal fibroblast (HDF) and VEGF-A in human vascular endothelial cells (HuVECs) were detected , and the effect of BWDs on acute wounds in mice was detected . Collagen and angiogenesis were evaluated through hematoxylin-eosin staining and Masson staining. The expressions of and and the activation of the Notch signaling pathway and were detected by quantitative reverse-transcriptase polymerase chain reaction.
The BCM had a nanoscale structure and provided a partial niche for the survival and proliferation of BMSCs. BWDs were successfully prepared and regulated the biological behaviors of wound healing-related cells and upregulated the expressions of in HDF and in HuVECs. BWDs promoted wound healing by increasing collagen type I synthesis and angiogenesis in acute wounds in mice.
BWDs prepared by the combination of nanomaterial BCMs and BMSCs facilitated acute wound healing, which may be regulated by activating the Notch signaling pathway.
细菌纤维素膜（BCM）作为一种用于伤口愈合的新型生物材料已得到广泛研究和应用，但频繁更换敷料会引起疼痛。局部应用骨髓间充质干细胞（BMSCs）需要一个微环境。此外，BCM与BMSCs联合应用的效果及机制尚未见报道。
通过孔隙率分析、扫描电子显微镜和傅里叶变换红外光谱对BCM进行形态学和化学鉴定。用BCM与BMSCs联合制备生物伤口敷料（BWDs）。检测BWDs对人皮肤成纤维细胞（HDF）和人血管内皮细胞（HuVECs）中VEGF-A的生物学作用，并检测BWDs对小鼠急性伤口的作用。通过苏木精-伊红染色和Masson染色评估胶原蛋白和血管生成情况。通过定量逆转录聚合酶链反应检测Notch信号通路相关基因的表达及激活情况。
BCM具有纳米级结构，为BMSCs的存活和增殖提供了部分微环境。成功制备了BWDs，其可调节伤口愈合相关细胞的生物学行为，并上调HDF中相关基因及HuVECs中VEGF-A的表达。BWDs通过增加I型胶原蛋白合成和促进小鼠急性伤口血管生成来促进伤口愈合。
纳米材料BCM与BMSCs联合制备的BWDs促进了急性伤口愈合，其机制可能是通过激活Notch信号通路来调节。
