Zheng Heng, Zhang Peng, Qin Jing, Guo Jiani, Deng Jun
Emergency Department of Hubei Third People's Hospital Affiliated to Jianghan University, Wuhan, Hubei Province, China.
School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan, China.
Front Microbiol. 2023 Jan 17;13:1092552. doi: 10.3389/fmicb.2022.1092552. eCollection 2022.
f.glauca () is a commonly used Chinese Medicinal Materials with great medicinal value. The medicinal plant and its endophytic bacteria are a symbiotic whole, and the endophytic bacteria are rich in species, and their metabolites are a treasure trove of natural compounds. However, there is a relative lack of analysis on the diversity, flora composition and network interactions of the endophytic bacteria of . In this study, high-throughput sequencing technology based on the Illumina Miseq platform was used to reveal the core microbiota by examining the diversity and community structures of tuber endophytic bacteria in grown under different regions and exploring the effect of region on its endophytic bacteria. Here, 1,265 endophytic ASVs were found to coexist with tuber in Guizhou and Hubei. At the phylum level, the dominant phyla were Proteobacteria, Actinobacteria and Acdobacteriota. At the family level, the dominant family were Comamonadaceae, Nocardicaece, Xanthobacteraceae, and Burkholderiaceae. At the genus level, and were represented the core microbiota in tuber, which served as the dominant genera that coexisted in all samples tested. Moreover, we found that the beta diversity of endophytic bacteria in tuber was higher level in the Guizhou region than Hubei region. Overall, this study results to provide a reference for screening active strains and interaction between plants and endophytic bacteria.
glauca（）是一种常用的中药材，具有很高的药用价值。药用植物与其内生细菌是一个共生整体，内生细菌种类丰富，其代谢产物是天然化合物的宝库。然而，对于 的内生细菌的多样性、菌群组成和网络相互作用的分析相对较少。在本研究中，基于Illumina Miseq平台的高通量测序技术被用于通过检测不同地区种植的 块茎内生细菌的多样性和群落结构来揭示核心微生物群，并探索地区对其内生细菌的影响。在此，发现1265个内生ASV与贵州和湖北的 块茎共存。在门水平上，优势门为变形菌门、放线菌门和酸杆菌门。在科水平上，优势科为丛毛单胞菌科、诺卡氏菌科、黄杆菌科和伯克霍尔德菌科。在属水平上， 和 代表了 块茎中的核心微生物群，它们是所有测试样本中共存的优势属。此外，我们发现 块茎内生细菌的β多样性在贵州地区高于湖北地区。总体而言，本研究结果为筛选活性菌株以及植物与内生细菌之间的相互作用提供了参考。
