Yu Xinrong, Gao Zichun, Mu Jiaxiang, Lian He, Meng Zhaoxu
Faculty of Medical Instrument, Shenyang Pharmaceutical University, Shenyang 110016, China.
Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China.
Biomater Sci. 2023 Mar 14;11(6):2158-2166. doi: 10.1039/d2bm01767a.
Blood coagulation is the body's main defense to bleeding caused by trauma and is divided into endogenous and exogenous pathways. Calcium ions play a very important role in the process of blood coagulation, as the ions activate the many enzymes that are required for coagulation. In this paper, gelatin hemostatic membranes containing calcium ions were prepared by electrospinning. The fibers were characterized with scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The biocompatibility and coagulation processes using the calcium ion-containing gelatin fibrous membranes were evaluated with dynamic whole-blood coagulation tests, hemolysis tests, coagulation time tests, and platelet adhesion tests. It was demonstrated that the calcium ion-containing gelatin membranes had lower hemolysis rates and shorter clotting times than commercially available hemostatic sponges and hemostatic gauzes. hemostasis experiments were also conducted on the tail vein and liver of mice. Animal experiments demonstrated that the incorporation of calcium ions into the electrospun gelatin membranes promoted platelet aggregation, ensured adhesion of the electrospun membrane to the wound and reduced the bleeding volume and hemostasis time. The composite calcium ion-gelatin electrospun membranes exhibited good and hemostatic abilities and accelerated blood clotting by stimulating the coagulation pathway to promote platelet aggregation at the wounds and the formation of mature blood clots for a new approach for acute trauma treatment.
血液凝固是人体对创伤出血的主要防御机制，分为内源性和外源性途径。钙离子在血液凝固过程中起着非常重要的作用，因为这些离子会激活凝血所需的多种酶。在本文中，通过静电纺丝制备了含钙离子的明胶止血膜。采用扫描电子显微镜、傅里叶变换红外光谱和X射线衍射对纤维进行了表征。使用含钙离子的明胶纤维膜的生物相容性和凝血过程通过动态全血凝固试验、溶血试验、凝血时间试验和血小板黏附试验进行了评估。结果表明，含钙离子的明胶膜的溶血率低于市售止血海绵和止血纱布，凝血时间更短。还对小鼠的尾静脉和肝脏进行了止血实验。动物实验表明，将钙离子掺入静电纺丝明胶膜中可促进血小板聚集，确保静电纺丝膜与伤口的黏附，并减少出血量和止血时间。复合钙离子-明胶静电纺丝膜具有良好的止血能力，通过刺激凝血途径促进伤口处血小板聚集和形成成熟血凝块，从而加速血液凝固，为急性创伤治疗提供了一种新方法。
