College of Environment and Resources, Xiangtan University, Xiangtan, 411105, Hunan, China.
Hunan Institute of Advanced Sensing and Information Technology, Xiangtan University, Xiangtan, 411105, Hunan, China.
Anal Chim Acta. 2023 Mar 8;1245:340861. doi: 10.1016/j.aca.2023.340861. Epub 2023 Jan 21.
Designing highly active nanozymes for bioanalysis and environmental sensing remains a challenge. In this study, transition metal, palladium (Pd) and iron (Fe), doped germanium oxide (GeO) nanozyme was designed and optimized. Compared with the pristine GeO nanozyme, the transition metal doped GeO nanozyme have lower Michaelis-Menten constants and higher catalytic activity, indicating that the Pd and Fe doped GeO nanozyme not only enhance their affinity for the substrate but also improve its catalytic activity. In addition, a colorimetric sensor based on the GeO@Pd-HO-TMB system was constructed for the visual detection of simazine in water samples due to the good affinity between TMB and simazine. This sensor has good selectivity and sensitivity with a detection limit of 6.21 μM because of the highest catalytic performance of GeO@Pd nanozyme. This study broadens the application of nanozymes in environmental field and other nanozymes can also be enhanced in activity by simple transition metal doping.
设计具有高活性的纳米酶用于生物分析和环境传感仍然是一个挑战。在这项研究中，设计并优化了过渡金属钯（Pd）和铁（Fe）掺杂的氧化锗（GeO）纳米酶。与原始的 GeO 纳米酶相比，过渡金属掺杂的 GeO 纳米酶具有更低的米氏常数和更高的催化活性，这表明 Pd 和 Fe 掺杂的 GeO 纳米酶不仅增强了它们对底物的亲和力，而且提高了其催化活性。此外，由于 TMB 与西玛津之间良好的亲和力，构建了基于 GeO@Pd-HO-TMB 体系的比色传感器，用于水样中西玛津的可视化检测。由于 GeO@Pd 纳米酶具有最高的催化性能，该传感器具有良好的选择性和灵敏度，检测限为 6.21 μM。本研究拓宽了纳米酶在环境领域的应用，并且通过简单的过渡金属掺杂也可以提高其他纳米酶的活性。
