由具有过氧化物酶特性的苦瓜多糖稳定的钯纳米颗粒用于过氧化氢检测。

Pd nanoparticles stabilized by bitter gourd polysaccharide with peroxidase properties for HO detection.

作者信息

Li Ruyu, Zhao Yu, Zhang Tingting, Ju Zejin, Ji Xianbing, Cui Yanshuai, Wang Longgang, Xiao Haiyan

机构信息

Hebei Key Laboratory of Nano-biotechnology, Hebei Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China; State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China.

Hebei Key Laboratory of Nano-biotechnology, Hebei Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.

出版信息

Int J Biol Macromol. 2023 Apr 1;233:123513. doi: 10.1016/j.ijbiomac.2023.123513. Epub 2023 Feb 3.

DOI:10.1016/j.ijbiomac.2023.123513

PMID:36739057

Abstract

The development of nanozymes using noble metal nanoparticles to replace natural peroxidase in bio-related detection has been gain great interest. Noble metal nanoparticles with small size have large specific surface area. However, small noble metal nanoparticles tend to aggregate without stabilizer. In this paper, small Pd nanoparticles (3-6 nm) stabilized by bitter gourd polysaccharide (Pd-BGP NPs) were prepared by using bitter gourd polysaccharide as reducing agent and stabilizing agent. Pd-BGP NPs had peroxidase-like catalytic property. And the catalytic kinetics of Pd-BGP NPs towards substrates conformed to the Michaelis-Menten equation. Furthermore, a method was established to detect HO using Pd-BGP NPs. The linear range and detection limit of this method was 20-320 μM and 2.04 μM, respectively. Finally, Pd-BGP NPs had good biocompatibility when the concentration was less than 80 μg/mL. The prepared Pd nanoparticles with high stability showed their good prospect in HO detection.

摘要

利用贵金属纳米颗粒替代生物相关检测中的天然过氧化物酶来开发纳米酶已引起了极大的关注。尺寸小的贵金属纳米颗粒具有大的比表面积。然而，小的贵金属纳米颗粒在没有稳定剂的情况下容易聚集。在本文中，以苦瓜多糖为还原剂和稳定剂制备了由苦瓜多糖稳定的小尺寸钯纳米颗粒（3 - 6纳米）（Pd - BGP NPs）。Pd - BGP NPs具有类过氧化物酶催化特性。并且Pd - BGP NPs对底物的催化动力学符合米氏方程。此外，建立了一种使用Pd - BGP NPs检测HO的方法。该方法的线性范围和检测限分别为20 - 320μM和2.04μM。最后，当浓度小于80μg/mL时，Pd - BGP NPs具有良好的生物相容性。所制备的具有高稳定性的钯纳米颗粒在HO检测中显示出良好的前景。