Foote Andrea, Schutz Kristin, Zhao Zirui, DiGianivittorio Pauline, Korwin-Mihavics Bethany R, LiPuma John J, Wargo Matthew J
Cellular, Molecular, and Biomedical Sciences Graduate Program, University of Vermont Larner College of Medicine, Burlington, Vermont, USA.
Department of Microbiology and Molecular Genetics, University of Vermont Larner College of Medicine, Burlington, Vermont, USA.
Microbiol Spectr. 2023 Feb 6;11(2):e0410522. doi: 10.1128/spectrum.04105-22.
Ralstonia insidiosa and Chryseobacterium gleum are bacterial species commonly found in potable water systems, and these two species contribute to the robustness of biofilm formation in a model six-species community from the International Space Station (ISS) potable water system. Here, we set about characterizing the interaction between these two ISS-derived strains and examining the extent to which this interaction extends to other strains and species in these two genera. The enhanced biofilm formation between the ISS strains of and is robust to starting inoculum and temperature and occurs in some but not all tested growth media, and evidence does not support a soluble mediator or coaggregation mechanism. These findings shed light on the ISS and interaction, though such enhancement is not common between these species based on our examination of other and strains, as well as other species of and . Thus, while the findings presented here increase our understanding of the ISS potable water model system, not all our findings are broadly extrapolatable to strains found outside of the ISS. Biofilms present in drinking water systems and terminal fixtures are important for human health, pipe corrosion, and water taste. Here, we examine the enhanced biofilm of cocultures for two very common bacteria from potable water systems: Ralstonia insidiosa and Chryseobacterium gleum. While strains originally isolated on the International Space Station show enhanced dual-species biofilm formation, terrestrial strains do not show the same interaction properties. This study contributes to our understanding of these two species in both dual-culture and monoculture biofilm formation.
阴险罗尔斯通氏菌和黏金黄杆菌是在饮用水系统中常见的细菌物种，这两个物种有助于在来自国际空间站（ISS）饮用水系统的一个模型六种群落中形成强大的生物膜。在此，我们着手表征这两种源自国际空间站的菌株之间的相互作用，并研究这种相互作用扩展到这两个属中的其他菌株和物种的程度。国际空间站的罗尔斯通氏菌和金黄杆菌菌株之间增强的生物膜形成对起始接种量和温度具有抗性，并且在一些但并非所有测试的生长培养基中都会发生，而且证据不支持可溶性介质或共聚集机制。这些发现揭示了国际空间站的罗尔斯通氏菌和金黄杆菌之间的相互作用，不过基于我们对其他罗尔斯通氏菌和金黄杆菌菌株以及罗尔斯通氏菌属和金黄杆菌属的其他物种的研究，这种增强在这些物种之间并不常见。因此，虽然此处呈现的发现增进了我们对国际空间站饮用水模型系统的理解，但并非所有我们的发现都能广泛外推至国际空间站之外发现的菌株。 存在于饮用水系统和终端装置中的生物膜对人类健康、管道腐蚀和水的味道很重要。在此，我们研究了来自饮用水系统的两种非常常见的细菌——阴险罗尔斯通氏菌和黏金黄杆菌——共培养物增强的生物膜。虽然最初在国际空间站分离的菌株显示出增强的双物种生物膜形成，但陆生菌株并未表现出相同的相互作用特性。这项研究有助于我们理解这两个物种在双培养和单培养生物膜形成中的情况。
