Wang Di, Wu Yong, Liu Yang, Liu Bilin, Gao Yuxin, Yang Yuxue, Zhang Yan, Liu Chen, Huo Yingzhe, Tang Aoyu, Xu Yunping, Wei Yuli
College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, PR China.
State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, PR China.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005615.
A Gram-stain-negative, non-motile and rod-shaped bacterium, designated as strain W52, was isolated from deep seawater of the Mariana Trench and characterized phylogenetically and phenotypically. The strain could grow at 10-47 °C (optimum 32 °C), at pH 5.0-8.0 (optimum 6.0) and with 0-9% NaCl (optimum 3 %, w/v). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that W52 was related to members of the genus and shared the highest identity with 501str8 (99.0 %), followed by JCM 11811, DSM 13258, 40DY170, KCTC 23501 and 12C25 with 97.0-98.8 % sequence similarity. 16S rRNA gene sequence identities between W52 and other members of the genus were below 97.0 %. The major respiratory quinone was MK-6. The polar lipids were phosphatidylethanolamine (PE), one unidentified aminolipid and three unidentified lipids. The strain had iso-C, iso-C 3-OH and iso-CG as the major fatty acids. The G+C content of the genomic DNA was 41.7 %. The combined genotypic and phenotypic data indicated that strain W52 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain W52 (=MCCC 1K05111= KCTC 82315).
从马里亚纳海沟的深层海水中分离出一株革兰氏阴性、无运动性的杆状细菌，命名为菌株W52，并对其进行了系统发育和表型特征分析。该菌株能在10-47°C（最适温度32°C）、pH 5.0-8.0（最适pH 6.0）和0-9% NaCl（最适3%，w/v）的条件下生长。基于16S rRNA基因序列的系统发育分析结果表明，W52与该属的成员相关，与501str8的同一性最高（99.0%），其次是JCM 11811、DSM 13258、40DY170、KCTC 23501和12C25，序列相似性为97.0-98.8%。W52与该属其他成员之间的16S rRNA基因序列同一性低于97.0%。主要呼吸醌为MK-6。极性脂质为磷脂酰乙醇胺（PE）、一种未鉴定的氨基脂质和三种未鉴定的脂质。该菌株以异-C、异-C 3-OH和异-CG作为主要脂肪酸。基因组DNA的G+C含量为41.7%。综合基因型和表型数据表明，菌株W52代表该属的一个新物种，为此提出新物种名为sp. nov.，模式菌株为W52（=MCCC 1K05111=KCTC 82315）。
