Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, Netherlands.
Department of Immunobiology, Yale University School of Medicine, New Haven, USA.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005635.
As part of a culturomics study to identify bacterial species associated with inflammatory bowel disease, a large collection of bacteria was isolated from patients with ulcerative colitis. Two of these isolates were tentatively identified as members of the family . Following phylogenetic analysis based on 16S rRNA gene sequence and genome sequences, both strain 128 and 539 were found to be most closely related to , with G+C contents of 48.6 and 50.5 mol%, respectively, and the genome sizes of 2 864 314 and 2 580 362 base pairs, respectively. Strains 128 and 539 were strict anaerobe rods that grew in long chains between 37 and 42 °C. Scanning electron microscopy did not reveal flagella, fimbriae or visible endospores. Biochemical analysis showed nearly identical results for both strains with enzymatic activity of C4 and C8 esterases, acid phosphatase, naphthol-AS-BI-phosphohydrolase, β-glucuronidase, -acetyl-β-glucosaminidase and arginine arylamidase. In addition, both strains produced indole and reduced nitrate. Major fatty acids were identified as C 9 (oleic acid, 64.06% in 128 and 74.35% in 539), C 7/C 9t/C 12t/UN17.834 (16.18 % in 128 and 6.22% in 539) and C (6.23% in 128 and 7.37% in 538). Based on these analyses two novel species are proposed, sp. nov. with the type strain 128 (=NCTC 14626=DSM 112815) and sp. nov. with the type strain 539 (=NCTC 14627=DSM 112814).
作为一项文化组学研究的一部分，旨在鉴定与炎症性肠病相关的细菌物种，从溃疡性结肠炎患者中分离出大量细菌。其中两种分离株被初步鉴定为 家族的成员。基于 16S rRNA 基因序列和基因组序列的系统发育分析，菌株 128 和 539 与 最为密切相关，GC 含量分别为 48.6%和 50.5%，基因组大小分别为 2864314 和 2580362 个碱基对。菌株 128 和 539 是严格的厌氧菌，在 37 至 42°C 之间以长链形式生长。扫描电子显微镜未显示鞭毛、菌毛或可见的芽孢。生化分析显示两种菌株的结果几乎完全相同，均具有 C4 和 C8 酯酶、酸性磷酸酶、萘酚-AS-BI-磷酸水解酶、β-葡萄糖醛酸酶、-乙酰-β-葡糖胺酶和精氨酸芳基酰胺酶的活性。此外，两种菌株均能产生吲哚和还原硝酸盐。主要脂肪酸鉴定为 C9（油酸，在 128 中占 64.06%，在 539 中占 74.35%）、C7/C9t/C12t/UN17.834（在 128 中占 16.18%，在 539 中占 6.22%）和 C （在 128 中占 6.23%，在 538 中占 7.37%）。基于这些分析，提出了两个新的物种， sp. nov.，其模式菌株为 128（=NCTC 14626=DSM 112815）和 sp. nov.，其模式菌株为 539（=NCTC 14627=DSM 112814）。
