Prapa Ioanna, Kompoura Vasiliki, Pavlatou Chrysoula, Nelios Grigorios, Mitropoulou Gregoria, Kostomitsopoulos Nikolaos, Plessas Stavros, Bezirtzoglou Eugenia, Karathanos Vaios T, Yanni Amalia E, Kourkoutas Yiannis
Laboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology and Genetics, Democritus University of Thrace, Dragana, 68100 Alexandroupolis, Greece.
Laboratory Animal Facility, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece.
Microorganisms. 2024 Oct 2;12(10):2004. doi: 10.3390/microorganisms12102004.
The present study aimed to investigate the effect of a dietary intervention including free or immobilized cells of the presumptive probiotic ORE5 on Corinthian currants, a food with beneficial impact in the condition of Type-1 Diabetes Mellitus (T1DM), on the microbiome composition of STZ-induced diabetic rats. Twenty four male Wistar rats were divided into four groups ( = 6 per group): healthy animals, which received the free (H_FP) or the immobilized ORE5 cells (H_IPC), and diabetic animals, which received the free (D_FP) or the immobilized ORE5 cells(D_IPC) for 4 weeks (10 cfu/day, in all groups). At the end of the dietary intervention, the D_IPC group exerted a lower concentration of the inflammatory cytokine IL-1 beta compared to D_FP. Consumption of immobilized ORE5 cells on Corinthian currants by diabetic animals led to increased loads of fecal lactobacilli and lower , coliforms, and levels, while Actinobacteria phylum, , and genera abundances were increased, and fecal lactic acid was elevated. Overall, the results of the present research demonstrated that functional ingredients could ameliorate gut dysbiosis present in T1DM and could be used to design dietary patterns aiming at T1DM management. However, well-designed clinical trials are necessary, in order to confirm the beneficial effects in humans.
本研究旨在调查一种饮食干预措施的效果，该措施包括将假定的益生菌ORE5的游离或固定化细胞添加到科林斯葡萄中（科林斯葡萄是一种对1型糖尿病（T1DM）病情有有益影响的食物），对链脲佐菌素诱导的糖尿病大鼠微生物群组成的影响。将24只雄性Wistar大鼠分为四组（每组n = 6）：健康动物组，分别接受游离的ORE5细胞（H_FP）或固定化的ORE5细胞（H_IPC）；糖尿病动物组，分别接受游离的ORE5细胞（D_FP）或固定化的ORE5细胞（D_IPC），持续4周（所有组均为每天10 cfu）。在饮食干预结束时，与D_FP组相比，D_IPC组的炎症细胞因子IL-1β浓度较低。糖尿病动物食用添加了固定化ORE5细胞的科林斯葡萄后，粪便中乳酸杆菌数量增加，大肠杆菌和肠球菌水平降低，而放线菌门、双歧杆菌属和阿克曼菌属的丰度增加，粪便乳酸含量升高。总体而言，本研究结果表明，功能性成分可以改善T1DM中存在的肠道菌群失调，可用于设计旨在管理T1DM的饮食模式。然而，需要设计良好的临床试验来证实对人类的有益效果。
