Department of Dental Surgery, Periodontology and Oral Mucosa Diseases, Poznan University of Medical Sciences, 70, Bukowska St., 60-812 Poznań, Poland.
Department of Dermatology, Poznan University of Medical Sciences, 49, Przybyszewskiego St., 61-701 Poznań, Poland.
Int J Environ Res Public Health. 2023 Jan 31;20(3):2524. doi: 10.3390/ijerph20032524.
Dermatitis herpetiformis (Duhring's disease, DH) is a chronic blistering cutaneous condition with pruritic polymorphic lesions, consisting of vesicles, papules or nodules and erythema, found predominantly on the extensor surfaces of the limbs, buttocks, and neck. Diagnosis is based on characteristic clinical and immunopathological findings. Oral manifestations of DH have rarely been described. The aim of the study was to evaluate IgA, IgG, IgM and C3 complement deposits in the oral mucosa in DH patients. Direct immunofluorescence (DIF) was performed on the oral mucosa specimens collected from 10 DH patients. Biopsy was taken in a local anesthesia from perilesional site from the buccal mucosa and then preserved in a standard procedure using polyclonal rabbit IgG, IgA, IgM and C3 antibodies. Granular IgA and C3 deposits were found in 6 patients (60%), and in 3 subjects (30%) the result was indeterminate. Significant fluorescence of the deposits along the basement membrane was observed in 2 patients, moderate fluorescence in 3 patients, and in 4 cases the result was indeterminate. C3 deposits were found in 5 subjects (50%), 3 of them being moderate and 2 indeterminate. No IgM and IgG deposits were detected in the collected buccal mucosa specimens.
疱疹样皮炎（Duhring 病，DH）是一种慢性水疱性皮肤病，伴有瘙痒性多形性病变，包括水疱、丘疹或结节和红斑，主要发生在四肢、臀部和颈部的伸展面。诊断基于特征性的临床和免疫病理学发现。DH 的口腔表现很少被描述。本研究旨在评估 DH 患者口腔黏膜中的 IgA、IgG、IgM 和 C3 补体沉积。对 10 例 DH 患者的口腔黏膜标本进行直接免疫荧光（DIF）检测。在局部麻醉下从颊黏膜的病变周围部位取活检，然后按照标准程序用多克隆兔 IgG、IgA、IgM 和 C3 抗体保存。6 例患者（60%）发现颗粒状 IgA 和 C3 沉积，3 例患者（30%）结果不确定。在 2 例患者中观察到沿基底膜的沉积有显著荧光，在 3 例患者中有中度荧光，在 4 例患者中结果不确定。5 例患者（50%）发现 C3 沉积，其中 3 例为中度，2 例为不确定。在收集的颊黏膜标本中未检测到 IgM 和 IgG 沉积。
