Steindler D A, Cooper N G
Department of Anatomy and Neurobiology, College of Medicine, University of Tennessee, Memphis 38163.
Brain Res. 1987 Nov;433(1):27-38. doi: 10.1016/0165-3806(87)90062-9.
The localization of glycosylated molecules and glia has been studied during early postnatal development in the mouse central nervous system (CNS) using autoradiographic detection of radiolabeled fucose incorporation, and in sections processed either for histochemistry or immunocytochemistry following binding of labeled lectins or an antibody to glial fibrillary acidic protein. Radiolabeled sugar incorporation, lectin binding of glycoconjugates, and glial labeling all reveal borders between nuclei within the diencephalon, midbrain, and brainstem through the first postnatal week. Glycoconjugate and glial boundaries exist throughout the CNS during pattern formation events, and they also are seen in relation to fine aspects of developing functional organization within individual structures (e.g. segmentation associated with the representation of mystacial vibrissae within the brainstem trigeminal complex). The observation that each of the probes employed in this study fails to label boundary organization during later postnatal times suggests that the distribution and chemistry of the glial/glycoconjugate network are dynamic, and they change in accordance with distinct maturational states of the nervous system.
利用放射自显影检测放射性标记岩藻糖的掺入，并在结合标记凝集素或抗胶质纤维酸性蛋白抗体后进行组织化学或免疫细胞化学处理的切片中，研究了小鼠中枢神经系统（CNS）出生后早期发育过程中糖基化分子和神经胶质的定位。放射性标记糖的掺入、糖缀合物的凝集素结合以及神经胶质标记在出生后的第一周内均揭示了间脑、中脑和脑干内各核之间的边界。在模式形成过程中，糖缀合物和神经胶质边界存在于整个中枢神经系统中，并且在与各个结构内发育中的功能组织的细微方面相关的情况下也能看到（例如与脑干三叉神经复合体中触须代表相关的分割）。本研究中使用的每种探针在出生后后期均未能标记边界组织，这一观察结果表明神经胶质/糖缀合物网络的分布和化学性质是动态的，并且它们会根据神经系统的不同成熟状态而变化。
