Institute of Horticulture, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, China.
Henan International Joint Laboratory of Crop Gene Resources and Improvement, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
Int J Mol Sci. 2023 Jul 24;24(14):11850. doi: 10.3390/ijms241411850.
Clubroot is a soil-borne disease caused by , which can seriously affect the growth and production of cruciferous crops, especially Chinese cabbage crops, worldwide. At present, few studies have been conducted on the molecular mechanism of this disease's resistance response. In this experiment, we analyzed the bioinformation of bra-miR167a, constructed a silencing vector (STTM167a) and an overexpression vector (OE-miR167a), and transformed them to to confirm the role of miR167a in the clubroot resistance mechanism of . Afterwards, phenotype analysis and expression level analysis of key genes were conducted on transgenic plants. From the result, we found that the length and number of lateral roots of silence transgenic STTM167a was higher than that of WT and OE-miR167a. In addition, the STTM167a transgenic induced up-regulation of disease resistance-related genes (, , , and ) at 3 days after inoculation. On the other hand, the auxin pathway genes (, , and ), which are involved in maintaining the balance of auxin/IAA and auxin response factor (), were down-regulated. These results indicate that bra-miR167a is negative to the development of lateral roots and auxins, but positive to the expression of resistance-related genes. This also means that the STTM167a can improve the resistance of clubroot by promoting lateral root development and the level of auxin, and can induce resistance-related genes by regulating its target genes. We found a positive correlation between miR167a and clubroot disease, which is a new clue for the prevention and treatment of clubroot disease.
根肿病是一种由引起的土传病害，可严重影响十字花科作物，尤其是白菜类作物的生长和生产，在世界各地。目前，对这种疾病的抗性反应的分子机制的研究很少。在本实验中，我们分析了 bra-miR167a 的生物信息，构建了沉默载体（STTM167a）和过表达载体（OE-miR167a），并将其转化为以确定 miR167a 在根肿病抗性机制中的作用。随后，对转基因植物进行了表型分析和关键基因的表达水平分析。从结果中，我们发现沉默型转基因 STTM167a 的侧根长度和数量均高于 WT 和 OE-miR167a。此外，STTM167a 转基因在接种后 3 天诱导了抗病相关基因（、、、和）的上调表达。另一方面，涉及维持生长素/IAA 和生长素响应因子（）平衡的生长素途径基因（、、和）下调。这些结果表明，bra-miR167a 对侧根和生长素的发育呈负相关，但对抗病相关基因的表达呈正相关。这也意味着 STTM167a 可以通过促进侧根发育和生长素水平来提高根肿病的抗性，并通过调节其靶基因来诱导抗病相关基因。我们发现 miR167a 与根肿病之间存在正相关关系，这为根肿病的防治提供了新的线索。
