bra-miR167a Targets and Negatively Regulates Immunity against .

Clubroot is a soil-borne disease caused by , which can seriously affect the growth and production of cruciferous crops, especially Chinese cabbage crops, worldwide. At present, few studies have been conducted on the molecular mechanism of this disease's resistance response. In this experiment, we analyzed the bioinformation of bra-miR167a, constructed a silencing vector (STTM167a) and an overexpression vector (OE-miR167a), and transformed them to to confirm the role of miR167a in the clubroot resistance mechanism of . Afterwards, phenotype analysis and expression level analysis of key genes were conducted on transgenic plants. From the result, we found that the length and number of lateral roots of silence transgenic STTM167a was higher than that of WT and OE-miR167a. In addition, the STTM167a transgenic induced up-regulation of disease resistance-related genes (, , , and ) at 3 days after inoculation. On the other hand, the auxin pathway genes (, , and ), which are involved in maintaining the balance of auxin/IAA and auxin response factor (), were down-regulated. These results indicate that bra-miR167a is negative to the development of lateral roots and auxins, but positive to the expression of resistance-related genes. This also means that the STTM167a can improve the resistance of clubroot by promoting lateral root development and the level of auxin, and can induce resistance-related genes by regulating its target genes. We found a positive correlation between miR167a and clubroot disease, which is a new clue for the prevention and treatment of clubroot disease.