Tam Khieu Thi, Thuy Nguyen Thi, Ngan Nguyen Thi Kim, Khai Nguyen Manh, Van Thanh Dang
Faculty of Chemistry, TNU-University of Sciences, Tan Thinh Ward, Thai Nguyen City, Vietnam.
Department of Environmental Engineering, International University, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam.
R Soc Open Sci. 2023 Feb 8;10(2):220819. doi: 10.1098/rsos.220819. eCollection 2023 Feb.
A green synthetic approach to synthesize silver nanoparticles (AgNPs) using the stem extract of for highly sensitive colorimetric detection of Hg with a low limit of detection of 23 nM and easy colorimetric read-out has been reported. In addition, the biosynthesized AgNPs demonstrated efficient antibacterial activity against , and . The morphology and structure of the as-synthesized AgNPs were examined using SEM, TEM, EDX, XRD and FT-IR analyses. The XRD and TEM results confirm the formation of AgNPs with an average particle size of 8-12 nm. The TLC, CC and HPLC revealed that four main compounds, pentacosanoic acid (), piperine (), -sitosterol (), and campesterol glucoside (), isolated from extract act as reducing and stabilizing agents for AgNP formation, and piperine plays a vital role in green synthesis. The chemical structures of these compounds were determined by ESI MS, FTIR, and one- and two-dimensional NMR spectroscopic data analysis. This approach is an efficient, green, cost-effective, eco-friendly and promising technique for synthesizing AgNPs with applications in the colorimetric detection of Hg and antibacterial activity.
已报道了一种绿色合成方法，即使用[植物名称]的茎提取物合成银纳米颗粒（AgNPs），用于高灵敏度比色法检测汞，检测限低至23 nM，且比色读出简便。此外，生物合成的AgNPs对[细菌名称1]、[细菌名称2]和[细菌名称3]表现出高效的抗菌活性。使用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、能量散射X射线光谱（EDX）、X射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）分析对合成的AgNPs的形态和结构进行了研究。XRD和TEM结果证实形成了平均粒径为8 - 12 nm的AgNPs。薄层色谱（TLC）、柱色谱（CC）和高效液相色谱（HPLC）显示，从[植物名称]提取物中分离出的四种主要化合物，二十五烷酸（[化合物英文名称1]）、胡椒碱（[化合物英文名称2]）、β-谷甾醇（[化合物英文名称3]）和菜油甾醇葡萄糖苷（[化合物英文名称4]），作为AgNP形成的还原剂和稳定剂，且胡椒碱在绿色合成中起关键作用。通过电喷雾电离质谱（ESI MS）、FTIR以及一维和二维核磁共振光谱数据分析确定了这些化合物的化学结构。这种方法是一种高效、绿色、经济高效、环保且有前景的技术，可用于合成具有汞比色检测和抗菌活性应用的AgNPs。
