Ultrasound-guided biopsy is widely used for disease detection and diagnosis. We plan to register preoperative imaging, such as positron emission tomography / computed tomography (PET/CT) and/or magnetic resonance imaging (MRI), with real-time intraoperative ultrasound imaging for improved localization of suspicious lesions that may not be seen on ultrasound but visible on other imaging modalities. Once the image registration is completed, we will combine the images from two or more imaging modalities and use Microsoft HoloLens 2 augmented reality (AR) headset to display three-dimensional (3D) segmented lesions and organs from previously acquired images and real-time ultrasound images. In this work, we are developing a multi-modal, 3D augmented reality system for the potential use in ultrasound-guided prostate biopsy. Preliminary results demonstrate the feasibility of combining images from multiple modalities into an AR-guided system.