Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, 77 Puhe Avenue, Shenbei New District, Shenyang, 110122, China.
Curr Neuropharmacol. 2023;21(9):1964-1979. doi: 10.2174/1570159X21666230216095938.
Exosomes, as nanoscale biological vesicles, have been shown to have great potential for biomedical applications. However, the low yield of exosomes limits their application. In this review, we focus on methods to increase exosome yield. Two main strategies are used to increase exosome production, one is based on genetic manipulation of the exosome biogenesis and release pathway, and the other is by pretreating parent cells, changing the culture method or adding different components to the medium. By applying these strategies, exosomes can be produced on a large scale to facilitate their practical application in the clinic.
外泌体作为纳米级生物囊泡，在生物医学应用方面具有巨大的潜力。然而，外泌体的产量低限制了它们的应用。在这篇综述中，我们专注于提高外泌体产量的方法。有两种主要策略可用于增加外泌体的产生，一种是基于对外泌体生物发生和释放途径的遗传操作，另一种是通过预处理亲代细胞、改变培养方法或向培养基中添加不同的成分。通过应用这些策略，可以大规模生产外泌体，从而促进它们在临床上的实际应用。
