Department of Neuroscience, Psychology, Drug Research and Child Health-NEUROFARBA-Pharmacology and Toxicology Section, University of Florence, Florence, Italy.
Department of Biochemistry-Functional Foods and Nutraceuticals Unit, School of Sciences, Federal University of Technology Akure, Akure, Nigeria.
Phytother Res. 2023 Jun;37(6):2381-2394. doi: 10.1002/ptr.7760. Epub 2023 Feb 20.
Osteoarthritis (OA) is a complex joint disease characterized by persistent pain. Unfortunately, current pharmacological therapies are unsatisfactory and characterized by side effects, reason why new strategies are needed. We tested the efficacy of different classes of compounds, ellagitannins and olean-type triterpenoids, contained in Anogeissus leiocarpus extract (Combretaceae family) in comparison to ellagitannins of Castanea sativa extract in a rat model of osteoarthritis induced by the intra-articular injection of sodium monoiodoacetate (MIA). The decoction of stem bark of A. leiocarpus AL-DEC-TOT (300 mg/kg; 4.8% triterpenoids; 11.0% tannins), the butanol extract AL-BuOH-EXT (120 mg/kg; triterpenoids 20.9%; tannins 6.4%) and its correlated aqueous residue AL-Res-H O (300 mg/kg; triterpenoids 0.7%; tannins 8.7%) and the decoction of C. sativa, CS-DEC-TOT, (240 mg/kg; triterpenoids 0.65%; tannins 10.8%) were orally administered for two weeks starting from the day of the damage. Behavioural tests highlighted that all stem bark extracts of A. leiocarpus counteracted hypersensitivity development, reduced spontaneous pain, and improved motor skills. Histologically, AL-DEC-TOT, AL-BuOH-EXT and AL-Res-H O were effective in preventing joint alterations. In conclusion, all the extracts were effective demonstrating that both olean-type triterpenoid and ellagitannin fractions have anti-hypersensitivity and restorative properties running the stem bark extracts of A. leiocarpus as a candidate in the treatment of OA.
骨关节炎（OA）是一种以持续性疼痛为特征的复杂关节疾病。不幸的是，目前的药物治疗效果不理想，且具有副作用，因此需要新的治疗策略。我们测试了不同类别的化合物，即在栎属（山毛榉科）提取物中含有的鞣花单宁和齐墩果烷型三萜类化合物，以及栗属（壳斗科）提取物中的鞣花单宁，在通过关节内注射单碘乙酸钠（MIA）诱导的骨关节炎大鼠模型中的疗效。栎属茎皮的煎剂 AL-DEC-TOT（300mg/kg；三萜类 4.8%；鞣质 11.0%）、正丁醇提取物 AL-BuOH-EXT（120mg/kg；三萜类 20.9%；鞣质 6.4%）及其相关的水提残余物 AL-Res-H O（300mg/kg；三萜类 0.7%；鞣质 8.7%）和栗属的煎剂 CS-DEC-TOT（240mg/kg；三萜类 0.65%；鞣质 10.8%），从损伤之日起，每天口服给药两周。行为测试表明，栎属的所有茎皮提取物均能对抗过敏反应的发展，减轻自发性疼痛，并改善运动技能。组织学检查表明，AL-DEC-TOT、AL-BuOH-EXT 和 AL-Res-H O 均能有效预防关节改变。综上所述，所有提取物均有效，证明齐墩果烷型三萜类和鞣花单宁类均具有抗敏和修复作用，栎属的茎皮提取物可作为治疗 OA 的候选药物。
