Shuaibu Mohammed N, Wuyep Ponchang T A, Yanagi Tetsuo, Hirayama Kenji, Ichinose Akitoyo, Tanaka Takashi, Kouno Isao
Natural Product Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki-shi, Japan.
Parasitol Res. 2008 Mar;102(4):697-703. doi: 10.1007/s00436-007-0815-1. Epub 2007 Dec 9.
The antitrypanosomal activity of methanolic extracts of Anogeissus leiocarpus and Terminalia avicennoides were evaluated in vitro against four strains of Trypanosoma species with minimum inhibitory concentration (MIC) value range of 12.5-50 mg/ml. Successive fractionations of the two plant extracts in water, butanol and ethyl acetate gave a range of activity (MIC, 20 to > or =50 microg/ml). Activity-guided and chromatographic analysis of butanolic fractions on Sephadex LH-20 column followed by high-performance liquid chromatography, nuclear magnetic resonance analysis and both ultraviolet and thin layer chromatography revealed hydrolysable tannins with a range of activity (MIC, 7.5-27.5 microg/ml or 14-91 microM). Effect of the compounds on fibroblasts did not reveal serious toxicity at moderate concentration but is concentration dependent.
对非洲臀果木和阿江榄仁甲醇提取物的抗锥虫活性进行了体外评估，受试对象为4种锥虫属菌株，最低抑菌浓度（MIC）值范围为12.5 - 50毫克/毫升。这两种植物提取物先后用水、丁醇和乙酸乙酯进行分级分离，得到了一系列活性（MIC，20至≥50微克/毫升）。对丁醇级分在葡聚糖凝胶LH - 20柱上进行活性导向和色谱分析，随后进行高效液相色谱、核磁共振分析以及紫外和薄层色谱分析，结果显示出具有一系列活性（MIC，7.5 - 27.5微克/毫升或14 - 91微摩尔）的可水解单宁。这些化合物对成纤维细胞的作用在中等浓度下未显示出严重毒性，但具有浓度依赖性。
