Shuaibu M N, Pandey K, Wuyep P A, Yanagi T, Hirayama K, Ichinose A, Tanaka T, Kouno I
Natural Product Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
Parasitol Res. 2008 Nov;103(6):1333-8. doi: 10.1007/s00436-008-1137-7. Epub 2008 Aug 9.
Stem barks of Anogeissus leiocarpus and Terminalia avicennoides widely used in Africa for treatment of some parasitic diseases were collected and made into methanolic extracts. The extracts were tested on four strains of promastigote forms of Leishmania in vitro. Solvent fractionation in aqueous, butanolic, and ethyl acetate layer indicated butanol and aqueous fractions to have a superior leishmanicidal activity. Chromatographic separation of the butanolic fraction on Sephadex LH-20 followed by nuclear magnetic resonance and correlation high-performance liquid chromatography revealed the presence of known hydrolyzable tannins and some related compounds-with castalagin as the major compound. The observed activity ranged from 62.5 to > or =150, 112.5 to > or =500, and 55 to >150 microg/ml for the crude methanolic extract, different solvent fractions, and the isolated compounds, respectively, on the four different Leishmania strains.
收集了在非洲广泛用于治疗某些寄生虫病的非洲臀果木（Anogeissus leiocarpus）和阿维森纳榄仁树（Terminalia avicennoides）的茎皮，并制成甲醇提取物。体外对四种利什曼原虫前鞭毛体菌株进行了提取物测试。水层、丁醇层和乙酸乙酯层的溶剂分级分离表明，丁醇和水层馏分具有较强的杀利什曼活性。丁醇馏分在Sephadex LH-20上进行色谱分离，随后进行核磁共振和相关高效液相色谱分析，结果显示存在已知的可水解单宁和一些相关化合物，其中主要化合物为栗木鞣花素。对于四种不同的利什曼原虫菌株，粗甲醇提取物、不同溶剂馏分和分离出的化合物的观察活性分别为62.5至≥150、112.5至≥500和55至>150微克/毫升。
