Liu Shuai, Tang Qingsong, Zhao Keji
Laboratory of Epigenome Biology, Systems Biology Center, Division of Intramural Research, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2023;2611:85-97. doi: 10.1007/978-1-0716-2899-7_7.
Spatial organization of the genome modulates pivotal biological processes. The emerging new technologies have provided novel insights into genome structure and its role in regulating cell activities. To examine the genome-wide chromatin interactions at accessible chromatin regions, we developed a DNA transposase-mediated analysis of chromatin looping (Trac-looping) method for simultaneously detecting chromatin interactions and chromatin accessibility. Here, we describe a detailed protocol of generating Trac-looping libraries.
基因组的空间组织调节关键的生物学过程。新兴的新技术为基因组结构及其在调节细胞活动中的作用提供了新的见解。为了检测可及染色质区域的全基因组染色质相互作用，我们开发了一种DNA转座酶介导的染色质环化分析（Trac-looping）方法，用于同时检测染色质相互作用和染色质可及性。在此，我们描述了生成Trac-looping文库的详细方案。
