Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, China.
School of Chinese Medicine, Southern Medical University, Guangzhou, China.
Front Immunol. 2021 Nov 19;12:774433. doi: 10.3389/fimmu.2021.774433. eCollection 2021.
Studies have shown that gut microbe disorder in mice due to early-life antibiotic exposure promotes glycolipid metabolism disorder in adulthood. However, the underlying mechanism remains unclear and there is not yet an effective intervention or treatment for this process.
The study investigated whether early-life azithromycin (AZT) exposure in mice could promote high-fat diet (HFD)-induced glycolipid metabolism disorder in adulthood. Moreover, the effect of citrus reticulata pericarpium (CRP) extract on glycolipid metabolism disorder regulation of gut microbiome in mice exposed to antibodies early in life were investigated.
Three-week-old mice were treated with AZT (50 mg/kg/day) drinking water for two weeks and then were fed a CRP diet (1% CRP extract) for four weeks and an HFD for five weeks. The results showed that early-life AZT exposure promoted HFD-induced glycolipid metabolism disorder, increased the levels of inflammatory factors, promoted the flora metabolism product trimethylamine N-oxide (TMAO), and induced microbial disorder in adult mice. Importantly, CRP extract mitigated these effects.
Taken together, these findings suggest that early-life AZT exposure increases the susceptibility to HFD-induced glycolipid metabolism disorder in adult mice, and CRP extract can decrease this susceptibility by regulating gut microbiome.
研究表明，由于早期生活中抗生素的暴露，小鼠的肠道微生物紊乱会促进成年后糖脂代谢紊乱。然而，其潜在机制尚不清楚，目前尚无针对该过程的有效干预或治疗措施。
本研究旨在探讨早期生活中阿奇霉素（AZT）暴露是否会促进高脂肪饮食（HFD）诱导的成年后糖脂代谢紊乱，并研究橙皮苷（CRP）提取物对早期暴露于抗体的小鼠肠道微生物群调节糖脂代谢紊乱的影响。
3 周龄的小鼠用 AZT（50mg/kg/天）饮用水处理 2 周，然后用 CRP 饮食（1% CRP 提取物）喂养 4 周，再用 HFD 喂养 5 周。结果表明，早期 AZT 暴露促进了 HFD 诱导的糖脂代谢紊乱，增加了炎症因子的水平，促进了菌群代谢产物三甲胺 N-氧化物（TMAO）的产生，并诱导了成年小鼠的微生物紊乱。重要的是，CRP 提取物减轻了这些作用。
综上所述，这些发现表明，早期 AZT 暴露会增加成年小鼠对 HFD 诱导的糖脂代谢紊乱的易感性，而 CRP 提取物通过调节肠道微生物群可以降低这种易感性。
