绵羊基因的表达及其多态性与饲料效率性状的关系。
Expression of the Ovine Gene and the Relationship Between Its Polymorphism and Feed Efficiency Traits.
作者信息
Zeng Xiwen, Wang Weimin, Zhang Deyin, Li Xiaolong, Zhang Yukun, Zhao Yuan, Zhao Liming, Wang Jianghui, Xu Dan, Cheng Jiangbo, Li Wenxin, Zhou Bubo, Lin Changchun, Yang Xiaobin, Zhai Rui, Ma Zongwu, Liu Jia, Cui Panpan, Weng Xiuxiu, Wu Weiwei, Zhang Xiaoxue, Zheng Wenxin
机构信息
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
The State Key Laboratory of Grassland Agroecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
出版信息
DNA Cell Biol. 2023 Apr;42(4):194-202. doi: 10.1089/dna.2022.0529. Epub 2023 Feb 24.
In the mutton industry, feed efficiency traits have the greatest influence on the economic benefits of sheep raised in housing conditions. In this study, quantitative real-time PCR (qRT-PCR), Sanger sequencing, and KASPar methods were used to detect the expression levels of the B cell scaffold protein with ankyrin repeats 1 () gene and the relationship between its polymorphism and feed efficiency traits in Hu sheep. The qRT-PCR results showed that the gene was extensively expressed in 10 tissues and it was expressed at remarkably higher levels in lymph than in other tissues ( < 0.05). Then, the polymorphism locus, g.93888 A > T, was detected in intron 4 of the gene and proved to be remarkably associated with feed efficiency traits ( < 0.05). Hence, the gene can be used as a candidate gene for improving the feed efficiency of Hu sheep and this locus could be used as a potential molecular marker for breeding high-feed efficiency sheep in future breeding efforts.
在肉羊产业中，饲料效率性状对舍饲绵羊的经济效益影响最大。本研究采用定量实时荧光定量PCR（qRT-PCR）、桑格测序和竞争性等位基因特异性PCR（KASPar）方法，检测了锚蛋白重复序列1（ANKRD1）基因的表达水平及其多态性与湖羊饲料效率性状之间的关系。qRT-PCR结果显示，ANKRD1基因在10种组织中广泛表达，且在淋巴组织中的表达水平显著高于其他组织（P<0.05）。随后，在ANKRD1基因的第4内含子中检测到多态性位点g.93888 A>T，且该位点与饲料效率性状显著相关（P<0.05）。因此，ANKRD1基因可作为提高湖羊饲料效率的候选基因，该位点可作为未来培育高饲料效率绵羊的潜在分子标记。
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