Gonçalves Vivian N, Lirio Juan M, Coria Silvia H, Lopes Fabyano A C, Convey Peter, de Oliveira Fábio S, Carvalho-Silva Micheline, Câmara Paulo E A S, Rosa Luiz H
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Instituto Antártico Argentino, 25 de Mayo 1143, General San Martín, Buenos Aires B1650HMK, Argentina.
Biology (Basel). 2023 Feb 9;12(2):275. doi: 10.3390/biology12020275.
We studied the fungal diversity present in soils sampled along a deglaciated chronosequence from para- to periglacial conditions on James Ross Island, north-east Antarctic Peninsula, using DNA metabarcoding. A total of 88 amplicon sequence variants (ASVs) were detected, dominated by the phyla , and . The uncommon phyla , , , and were detected. Unknown fungi identified at higher hierarchical taxonomic levels (Fungal sp. 1, Fungal sp. 2, sp. and sp.) and taxa identified at generic and specific levels ( sp., sp., , , , sp. and sp.) dominated the assemblages. In general, the assemblages displayed high diversity and richness, and moderate dominance. Only 12 of the fungal ASVs were detected in all chronosequence soils sampled. Sequences representing saprophytic, pathogenic and symbiotic fungi were detected. Based on the sequence diversity obtained, Clearwater Mesa soils contain a complex fungal community, including the presence of fungal groups generally considered rare in Antarctica, with dominant taxa recognized as cold-adapted cosmopolitan, endemic, saprotrophic and phytopathogenic fungi. Clearwater Mesa ecosystems are impacted by the effects of regional climatic changes, and may provide a natural observatory to understand climate change effects over time.
我们利用DNA宏条形码技术，研究了南极半岛东北部詹姆斯罗斯岛从准冰川到近冰川条件下沿冰川消退时间序列采集的土壤中的真菌多样性。共检测到88个扩增子序列变体（ASV），主要由门、和门主导。还检测到了不常见的门、、、和门。在较高分类等级上鉴定出的未知真菌（真菌物种1、真菌物种2、物种和物种）以及在属和种水平上鉴定出的分类群（物种、物种、、、、物种和物种）在群落中占主导地位。总体而言，群落表现出高多样性和丰富度，以及适度的优势度。在所有采样的时间序列土壤中仅检测到12个真菌ASV。检测到了代表腐生真菌、致病真菌和共生真菌的序列。根据获得的序列多样性，克利尔沃特台地土壤含有复杂的真菌群落，包括通常被认为在南极洲罕见的真菌类群，优势分类群被认为是适应寒冷的广布种、特有种、腐生真菌和植物病原真菌。克利尔沃特台地生态系统受到区域气候变化的影响，可能为了解长期气候变化影响提供一个自然观测站。
