Institute of Pesticides and Environmental Protection, Belgrade, Serbia.
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
J Environ Sci Health B. 2021;56(1):54-63. doi: 10.1080/03601234.2020.1838821. Epub 2020 Nov 6.
Twenty-two strains of spp. ( species complex [THSC], f. and ) causing green mold disease on edible mushrooms (button mushroom, shiitake and oyster mushroom), collected during 2004-2018 from four countries (Serbia, North Macedonia, Croatia, and Hungary) were examined. Based on their ITS (internal transcribed spacer) sequences, strains from shiitake mushroom in Serbia were identified as members of the THSC, while in samples obtained from Serbian and North-Macedonian oyster mushroom farms THSC, and were detected, which represent the first findings in the region. In fungicide susceptibility tests, all examined strains were found to be highly sensitive to prochloraz (ED<0.4 µg mL) and considerably susceptible to metrafenone (ED < 4 µg mL). The most sensitive taxon to both fungicides was THSC from oyster mushroom. The toxicity of metrafenone was satisfying and strains from oyster mushroom showed the highest sensitivity (ED < 1.43 µg mL), while strains originating from button mushroom and shiitake displayed similar susceptibilities (ED < 3.64 µg mL). After additional trials, metrafenone might also be recommended for the control of green mold disease in mushroom farms.
22 株 spp.（种复合体 [THSC]、 和 ）引起的绿霉病，食用蘑菇（香菇、香菇和牡蛎蘑菇），2004-2018 年从四个国家（塞尔维亚、北马其顿、克罗地亚和匈牙利）收集。基于其 ITS（内部转录间隔区）序列，来自塞尔维亚香菇的菌株被鉴定为 THSC 的成员，而在从塞尔维亚和北马其顿牡蛎蘑菇农场获得的样本中，检测到了 THSC、 和 ，这是该地区的首次发现。在杀菌剂敏感性试验中，所有检查的 菌株均对百菌清（ED<0.4 µg mL）高度敏感，对甲呋酰胺（ED < 4 µg mL）相当敏感。对这两种杀菌剂最敏感的分类群是来自牡蛎蘑菇的 THSC。甲呋酰胺的毒性令人满意，来自牡蛎蘑菇的菌株显示出最高的敏感性（ED < 1.43 µg mL），而来自香菇和香菇的菌株显示出相似的敏感性（ED < 3.64 µg mL）。经过进一步的试验，甲呋酰胺也可能被推荐用于控制蘑菇场的绿霉病。
