Sensitivity of strains from edible mushrooms to the fungicides prochloraz and metrafenone.

Twenty-two strains of spp. ( species complex [THSC], f. and ) causing green mold disease on edible mushrooms (button mushroom, shiitake and oyster mushroom), collected during 2004-2018 from four countries (Serbia, North Macedonia, Croatia, and Hungary) were examined. Based on their ITS (internal transcribed spacer) sequences, strains from shiitake mushroom in Serbia were identified as members of the THSC, while in samples obtained from Serbian and North-Macedonian oyster mushroom farms THSC, and were detected, which represent the first findings in the region. In fungicide susceptibility tests, all examined strains were found to be highly sensitive to prochloraz (ED<0.4 µg mL) and considerably susceptible to metrafenone (ED < 4 µg mL). The most sensitive taxon to both fungicides was THSC from oyster mushroom. The toxicity of metrafenone was satisfying and strains from oyster mushroom showed the highest sensitivity (ED < 1.43 µg mL), while strains originating from button mushroom and shiitake displayed similar susceptibilities (ED < 3.64 µg mL). After additional trials, metrafenone might also be recommended for the control of green mold disease in mushroom farms.