Department of Natural Polymers, Bioactive and Biocompatible Materials, "Petru Poni" Institute of Macromolecular Chemistry, 700487 Iasi, Romania.
Center of Advanced Research in Bionanoconjugates and Biopolymers, "Petru Poni" Institute of Macromolecular Chemistry, 700487 Iasi, Romania.
Int J Mol Sci. 2023 Feb 20;24(4):4213. doi: 10.3390/ijms24044213.
Current trends in the development of wound dressings are oriented towards the use of biopolymer-based materials, due to their unique properties such as non-toxicity, hydrophilicity, biocompatibility and biodegradability, properties that have advantageous therapeutic characteristics. In this regard, the present study aims to develop hydrogels based on cellulose and dextran (CD) and to reveal their anti-inflammatory performance. This purpose is achieved by incorporating plant bioactive polyphenols (PFs) in CD hydrogels. The assessments include establishing the structural characteristics using attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy, the morphology by scanning electron microscopy (SEM), the swelling degree of hydrogels, the PFs incorporation/release kinetics and the hydrogels' cytotoxicity, together with evaluation of the anti-inflammatory properties of PFs-loaded hydrogels. The results show that the presence of dextran has a positive impact on the hydrogel's structure by decreasing the pore size at the same time as increasing the uniformity and interconnectivity of the pores. In addition, there is an increased degree of swelling and of the encapsulation capacity of PFs, with the increase of the dextran content in hydrogels. The kinetics of PFs released by hydrogels was studied according to the Korsmeyer-Peppas model, and it was observed that the transport mechanisms depend on hydrogels' composition and morphology. Furthermore, CD hydrogels have been shown to promote cell proliferation without cytotoxicity, by successfully culturing fibroblasts and endothelial cells on CD hydrogels (over 80% viability). The anti-inflammatory tests performed in the presence of lipopolysaccharides demonstrate the anti-inflammatory properties of the PFs-loaded hydrogels. All these results provide conclusive evidence on the acceleration of wound healing by inhibiting the inflammation process and support the use of these hydrogels encapsulated with PFs in wound healing applications.
目前，伤口敷料的发展趋势倾向于使用基于生物聚合物的材料，因为它们具有独特的特性，如无毒、亲水性、生物相容性和可生物降解性，这些特性具有有利的治疗特性。在这方面，本研究旨在开发基于纤维素和葡聚糖 (CD) 的水凝胶，并揭示其抗炎性能。这一目的是通过将植物生物活性多酚 (PFs) 掺入 CD 水凝胶来实现的。评估包括使用衰减全反射傅里叶变换红外 (ATR-FTIR) 光谱建立结构特征、扫描电子显微镜 (SEM) 形貌、水凝胶的溶胀度、PFs 的掺入/释放动力学以及水凝胶的细胞毒性，同时评估载有 PFs 的水凝胶的抗炎性能。结果表明，葡聚糖的存在通过减小孔径的同时增加孔径的均匀性和连通性，对水凝胶的结构产生积极影响。此外，随着水凝胶中葡聚糖含量的增加，水凝胶的溶胀度和 PFs 的包封能力也增加。根据 Korsmeyer-Peppas 模型研究了水凝胶释放 PFs 的动力学，观察到运输机制取决于水凝胶的组成和形态。此外，CD 水凝胶已被证明可以促进细胞增殖而没有细胞毒性，通过在 CD 水凝胶上成功培养成纤维细胞和内皮细胞（超过 80%的活力）。在脂多糖存在下进行的抗炎测试证明了载有 PFs 的水凝胶的抗炎特性。所有这些结果都提供了确凿的证据，证明通过抑制炎症过程加速伤口愈合，并支持在伤口愈合应用中使用封装有 PFs 的这些水凝胶。
