Du Kun, Yang Yang, Li Jinping, Wang Ming, Jiang Jinjin, Wu Jian, Fang Yujie, Xiang Yang, Wang Youping
Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China.
Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550008, China.
Life (Basel). 2023 Jan 22;13(2):310. doi: 10.3390/life13020310.
Phosphorus stress is one of the important factors restricting plant growth and development, and the microRNA (miRNA) family is involved in the regulation of the response to plant nutrient stress by repressing the expression of target genes at the post-transcriptional or translational level. miR399 is involved in the transportation of phosphate in multiple plants by improving tolerance to low Pi conditions. However, the effect of miR399 on the response of low Pi stress in rapeseed ( L.) is unclear. The present study showed a significant increase in taproot length and lateral root number of plants overexpressing Bna-miR399c, while the biomass and Pi accumulation in shoots and roots increased, and the anthocyanin content decreased and chlorophyll content improved under low Pi stress. The results illustrate that Bna-miR399c could enhance the uptake and transportation of Pi in soil, thus making B. napus more tolerant to low Pi stress. Furthermore, we confirmed that is one of the targets of Bna-miR399c, and the rejection of Pi in rapeseed seedlings increased due to the overexpression of . Hence, we suggest that miR399c- module can effectively regulate the homeostasis of Pi in . Our study can also provide the theoretical basis for germplasm innovation and the design of intelligent crops with low nutrient input and high yield to achieve the dual objectives of income and yield increase and environmental protection in .
磷胁迫是限制植物生长发育的重要因素之一，而微小RNA（miRNA）家族通过在转录后或翻译水平抑制靶基因的表达参与植物对养分胁迫响应的调控。miR399通过提高对低磷条件的耐受性参与多种植物中磷的转运。然而，miR399对油菜低磷胁迫响应的影响尚不清楚。本研究表明，过表达Bna-miR399c的植株主根长度和侧根数量显著增加，同时在低磷胁迫下地上部和根部的生物量及磷积累增加，花青素含量降低，叶绿素含量提高。结果表明，Bna-miR399c可增强土壤中磷的吸收和转运，从而使甘蓝型油菜对低磷胁迫更具耐受性。此外，我们证实 是Bna-miR399c的靶标之一，且由于 的过表达，油菜幼苗对磷的排斥增加。因此，我们认为miR399c- 模块可有效调节 中磷的稳态。我们的研究还可为种质创新以及设计低养分投入、高产的智能作物提供理论依据，以实现油菜增产增收和环境保护的双重目标。
