Viscusi Gianluca, Paolella Gaetana, Lamberti Elena, Caputo Ivana, Gorrasi Giuliana
Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy.
Department of Chemistry and Biology "A. Zambelli", University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy.
Membranes (Basel). 2023 Feb 17;13(2):242. doi: 10.3390/membranes13020242.
Fibrous membranes of polycaprolactone (PCL)-polyvinylpyrrolidone (PVP) encapsulating 15% wt of quercetin are fabricated by a uniaxial electrospinning technique. Morphological analysis of the electrospun systems proved the fabrication of micrometric fibers (1.58 µm for PCL/PVP and 2.34 µm for quercetin-loaded membrane). The liquid retention degree of the electrospun membranes is evaluated by testing four different liquid media. The contact angle estimation is performed by testing three liquids: phosphate buffer solution, basic solution (pH = 13) and acidic solution (pH = 3), showing high hydrophobicity degree (contact angles > 90°) in all cases. The release of quercetin from the nanofibers in PBS (phosphate buffer solution) and pH = 3 medium, modeled through different models, shows the possibility of a fine tuning of drug release (up to 7 days) for the produced materials. The release profiles attained a plateau regime after roughly 50 h up to 82% and 71% for PBS and pH = 3 media, respectively. Then, since quercetin is known to undergo photooxidation upon UV radiation, release tests after different UV treatment times are carried out and compared with the untreated membrane, demonstrating that the release of the active drug changes from 82% for no-irradiated sample up to 57% after 10 h of UV exposure. The biology activity of released quercetin is evaluated on two human cell lines. The reported results demonstrate the ability of the quercetin-loaded membranes to reduce cell viability of human cell lines in two different conditions: direct contact between cells and quercetin-loaded membranes and cells treatment with culture medium previously conditioned with quercetin-loaded membranes. Therefore, the reported preliminary data confirm the possibility of applying the electrospun quercetin-loaded PCL-PVP membranes for health applications.
采用单轴静电纺丝技术制备了包裹15%（重量）槲皮素的聚己内酯（PCL）-聚乙烯吡咯烷酮（PVP）纤维膜。对静电纺丝体系的形态分析证明制备出了微米级纤维（PCL/PVP为1.58 µm，负载槲皮素的膜为2.34 µm）。通过测试四种不同的液体介质来评估静电纺丝膜的液体保留程度。通过测试三种液体进行接触角估算：磷酸盐缓冲溶液、碱性溶液（pH = 13）和酸性溶液（pH = 3），结果表明在所有情况下均具有高疏水性（接触角>90°）。通过不同模型对纳米纤维在PBS（磷酸盐缓冲溶液）和pH = 3介质中槲皮素的释放进行建模，结果表明所制备材料具有微调药物释放（长达7天）的可能性。在大约50小时后，PBS和pH = 3介质的释放曲线分别达到82%和71%的平稳状态。然后，由于已知槲皮素在紫外线辐射下会发生光氧化，因此进行了不同紫外线处理时间后的释放测试，并与未处理的膜进行比较，结果表明活性药物的释放从未辐照样品的82%变化到紫外线照射10小时后的57%。在两种人类细胞系上评估了释放的槲皮素的生物学活性。所报道的结果表明，负载槲皮素的膜在两种不同条件下能够降低人类细胞系的细胞活力：细胞与负载槲皮素的膜直接接触以及用先前用负载槲皮素的膜预处理的培养基处理细胞。因此，所报道的初步数据证实了将静电纺丝负载槲皮素的PCL - PVP膜应用于健康领域的可能性。
