Department of Food Science, Fu Jen Catholic University, New Taipei City 242, Taiwan.
National Institute of Environmental Health Sciences, Miaoli 350, Taiwan.
Molecules. 2023 Feb 8;28(4):1639. doi: 10.3390/molecules28041639.
The objective of this study was to develop a simultaneous analysis method of furan and its 10 derivatives in different food commodities. The results indicated that furan and its 10 derivatives could be separated within 9.5 min by using a HP-5MS column and gas chromatography-tandem mass spectrometry (GC-MS/MS) with multiple reaction monitoring mode for detection. Furthermore, this method could resolve several furan isomers, such as 2-methyl furan and 3-methyl furan, as well as 2,3-dimethyl furan and 2,5-dimethyl furan. The most optimal extraction conditions were: 5 g of the fruit or juice sample mixed with 5 mL of the saturated NaCl solution, separately, or 1 g of the canned oily fish sample mixed with 9 mL of the saturated NaCl solution, followed by the equilibration of each sample at 35 °C for 15 min, using a carboxen-polydimethylsiloxane SPME arrow to adsorb the analytes for 15 min at 35 °C for subsequent analysis by GC-MS/MS. For method validation of all the analytes in the different food matrices, the recovery was 76-117% and the limit of the quantitation was 0.003-0.675 ng/g, while the relative standard deviation (RSD%) of the intra-day variability range from 1-16%, and that of the inter-day variability was from 4-20%. The method validation data further demonstrated that a reliable method was established for the analysis of furan and its 10 derivatives in commercial foods.
本研究旨在开发一种同时分析不同食品中呋喃及其 10 种衍生物的方法。结果表明，采用 HP-5MS 柱和气相色谱-串联质谱（GC-MS/MS）多反应监测模式，可在 9.5 分钟内分离呋喃及其 10 种衍生物。此外，该方法还可分辨几种呋喃异构体，如 2-甲基呋喃和 3-甲基呋喃，以及 2,3-二甲基呋喃和 2,5-二甲基呋喃。最佳提取条件为：将 5 g 水果或果汁样品与 5 mL 饱和 NaCl 溶液混合，或 1 g 罐装油性鱼样品与 9 mL 饱和 NaCl 溶液混合，然后将每个样品在 35°C 下平衡 15 min，使用羧基-聚二甲基硅氧烷 SPME 箭头在 35°C 下吸附分析物 15 min，随后进行 GC-MS/MS 分析。对不同食品基质中所有分析物的方法验证，回收率为 76-117%，定量限为 0.003-0.675ng/g，日内变异度的相对标准偏差（RSD%）为 1-16%，日间变异度的 RSD%为 4-20%。方法验证数据进一步表明，已建立了一种可靠的方法，可用于分析商业食品中的呋喃及其 10 种衍生物。
