College of Life Science, Shandong Agricultural University, Taian 271018, PR China.
Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
Int J Biol Macromol. 2018 Dec;120(Pt B):1490-1499. doi: 10.1016/j.ijbiomac.2018.09.134. Epub 2018 Sep 25.
The aims of this work were to investigate the antioxidant, anti-hyperlipidemia and hepatic protection of Morehella esculenta polysaccharide (MPS) from fruiting body and its enzyme-assisted MPS (EnMPS). The in vitro scavenging rates of EnMPS at 600 mg/L on superoxide, hydroxyl and 1,1‑diphenyl‑2‑pyrazole hydrazide (DPPH) radicals were 76.92 ± 2.61%, 66.74 ± 2.56% and 75.78 ± 2.4%, higher than those of MPS, respectively. Animals experiments showed that the EnMPS exhibited superior abilities of reducing hepatic lipid levels by monitoring the serum enzyme activities (ALP, ALT, ALB and AST) and serum lipid levels (CK, TC, TG, HDL-C, LDL-C and LDH), enhancing the hepatic antioxidant enzymes (FFA, SOD, CAT and T-AOC) and decreasing the lipid peroxidation (MDA and MPO). The results suggested that the EnMPS can act as a natural candidate for developing drugs to reduce blood lipids, resist oxidation and protect the liver.
本研究旨在探究来自子实体的软枣猕猴桃多糖（MPS）及其酶解产物（EnMPS）的抗氧化、抗高血脂和肝保护作用。EnMPS 在 600mg/L 时对超氧阴离子、羟自由基和 1,1-二苯基-2-苦肼基自由基的体外清除率分别为 76.92±2.61%、66.74±2.56%和 75.78±2.4%，均高于 MPS。动物实验表明，通过监测血清酶活性（碱性磷酸酶、丙氨酸氨基转移酶、白蛋白和天冬氨酸氨基转移酶）和血清脂质水平（肌酸激酶、总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇和乳酸脱氢酶），EnMPS 具有降低肝脂质水平的能力，同时增强肝抗氧化酶（游离脂肪酸、超氧化物歧化酶、过氧化氢酶和总抗氧化能力），降低脂质过氧化（丙二醛和髓过氧化物酶）。结果表明，EnMPS 可作为开发降血脂、抗氧化和保肝药物的天然候选物。
