Zarzecki Micheli Stéfani, Araujo Stífani M, Bortolotto Vandreza C, de Paula Mariane Trindade, Jesse Cristiano Ricardo, Prigol Marina
Laboratório de Avaliações Farmacológicas e Toxicológicas aplicadas às Moléculas Bioativas - Unipampa, Universidade Federal do Pampa - Campus Itaqui, Itaqui, RS 97650-000, Brazil.
Toxicol Rep. 2014 May 12;1:200-208. doi: 10.1016/j.toxrep.2014.02.003. eCollection 2014.
Chrysin (5,7-dihydroxyflavone) is a flavonoid, natural component of traditional medicinal herbs, present in honey, propolis and many plant extracts. The objective of this study was to investigate the hypolipidemic properties of chrysin on Triton WR-1339-induced hyperlipidemia in female C57BL/6 mice. Triton WR-1339 was administered intraperitoneally (400 mg/kg) to overnight-fasted mice to develop acute hyperlipidemia. Chrysin was administered orally (10 mg/kg) 30 min before Triton WR-1339. At 24 h after Triton WR-1339 injection, blood samples were collected to measure plasma lipid levels. The hepatic thiobarbituric acid reactive substances (TBARS), carbonyl content, non-protein sulfhydryl (NPSH) and ascorbic acid (AA) levels, as well as catalase (CAT) and superoxide dismutase (SOD) activity were recorded. Chrysin administration significantly decreased total cholesterol levels. In addition, it partially decreased non-high density lipoprotein-cholesterol and triglycerides levels in plasma of hyperlipidaemic mice. In addition chrysin administration prevented the increase on TBARS levels and prevented the decrease in SOD activity induced by Triton WR-1339. These findings indicated that chrysin was able to decrease plasma lipids concentration and that its antioxidant properties was, at least in part, involved in the hypolipidaemic action of chrysin.
白杨素（5,7 - 二羟基黄酮）是一种黄酮类化合物，是传统草药中的天然成分，存在于蜂蜜、蜂胶和许多植物提取物中。本研究的目的是探讨白杨素对Triton WR - 1339诱导的雌性C57BL/6小鼠高脂血症的降血脂特性。将Triton WR - 1339腹腔注射（400 mg/kg）给禁食过夜的小鼠以诱发急性高脂血症。在注射Triton WR - 1339前30分钟口服给予白杨素（10 mg/kg）。在注射Triton WR - 1339后24小时，采集血样以测量血浆脂质水平。记录肝脏硫代巴比妥酸反应性物质（TBARS）、羰基含量、非蛋白巯基（NPSH）和抗坏血酸（AA）水平，以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性。给予白杨素显著降低了总胆固醇水平。此外，它部分降低了高脂血症小鼠血浆中非高密度脂蛋白胆固醇和甘油三酯水平。此外，给予白杨素可防止TBARS水平升高，并防止Triton WR - 1339诱导的SOD活性降低。这些发现表明，白杨素能够降低血浆脂质浓度，并且其抗氧化特性至少部分参与了白杨素的降血脂作用。
