Hessling Martin, Sicks Ben, Lau Bernhard
Institute of Medical Engineering and Mechatronics, Ulm University of Applied Sciences, Albert-Einstein-Allee 55, D-89081 Ulm, Germany.
Pathogens. 2023 Jan 29;12(2):213. doi: 10.3390/pathogens12020213.
(1) Background: Far-UVC radiation in the spectral range 200-230 nm has, according to previous findings, a strong antimicrobial effect on pathogens, but exhibits hardly any harmful effect on human skin. Therefore, the present study will discuss whether such radiation could also be suitable for hand disinfection in the healthcare sector. (2) Methods: Hands and gloves were microbially contaminated and exposed to radiation from a 222 nm krypton-chloride-excimer lamp. The applied doses were 23 mJ/cm and 100 mJ/cm, respectively. Irradiated and non-irradiated hands and gloves were pressed onto agar plates and colonies were counted and compared after 24 h of incubation. For comparison, we also treated hands and gloves with a commercial liquid alcohol-based disinfectant. (3) Results: On the hand, the 23 mJ/cm resulted in the reduction of the observed colonies on the agar plates by one log level. For the gloves irradiated with 100 mJ/cm, a colony reduction of 1.3 log levels was recorded. In the comparative experiments with the commercial disinfectant, a colony reduction of 1.9 and approximately one log level was observed on hand and gloves, respectively. (4) Conclusion: In both cases, far-UVC radiation provided a considerable reduction in microorganisms. However, compared to published far-UVC irradiation results in suspensions, the disinfection success on hands and gloves was rather low. With regard to the irradiation limits currently existing in the European Union, multiple daily hand disinfection with far-UVC radiation is actually legally not possible at present, but the thresholds are currently under discussion and could change in the future. Far-UVC disinfection of hands in gloves seems theoretically possible if attention is paid to potential perforations in the gloves.
(1) 背景：根据先前的研究结果，光谱范围在200 - 230 nm的远紫外线辐射对病原体具有很强的抗菌作用，但对人体皮肤几乎没有任何有害影响。因此，本研究将探讨这种辐射是否也适用于医疗保健领域的手部消毒。(2) 方法：对手部和手套进行微生物污染，并使其暴露于222 nm氪氯准分子灯的辐射下。施加的剂量分别为23 mJ/cm²和100 mJ/cm²。将辐照和未辐照的手部及手套按压在琼脂平板上，培养24小时后计数并比较菌落。为作比较，我们还用市售的酒精基液体消毒剂对手部和手套进行了处理。(3) 结果：手部接受23 mJ/cm²的辐射后，琼脂平板上观察到的菌落减少了一个对数级。对于接受100 mJ/cm²辐照的手套，记录到菌落减少了1.3个对数级。在与市售消毒剂的对比实验中，手部和手套上观察到的菌落减少分别为1.9个对数级和大约一个对数级。(4) 结论：在这两种情况下，远紫外线辐射都使微生物数量大幅减少。然而，与已发表的远紫外线在悬浮液中的辐照结果相比，手部和手套上的消毒效果相当低。就目前欧盟存在的辐照限值而言，目前实际上依法不可能每天多次使用远紫外线辐射对手部进行消毒，但这些限值目前正在讨论中，未来可能会改变。如果注意手套上的潜在穿孔情况，理论上对手套中的手部进行远紫外线消毒似乎是可行的。
