Ianchis Raluca, Alexa Rebeca Leu, Gifu Ioana Catalina, Marin Maria Minodora, Alexandrescu Elvira, Constantinescu Roxana, Serafim Andrada, Nistor Cristina Lavinia, Petcu Cristian
National Research & Development Institute for Chemistry and Petrochemistry, ICECHIM, Spl. Independentei No. 202, 6th District, 060021 Bucharest, Romania.
Advanced Polymer Materials Group, Politehnica University of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania.
Pharmaceutics. 2023 Jan 21;15(2):373. doi: 10.3390/pharmaceutics15020373.
Salecan, a kind of polysaccharide, is produced by the Agrobacterium ZX09 salt tolerant strain. In this study, green crosslinked citric acid-salecan hydrogels are explored as novel materials with a high potential for use in regenerative medicine. The impact of salecan and citric acid on the final crosslinked hydrogels was intensively studied and estimated in terms of the whole physicochemical properties and antimicrobial activity. FTIR spectra demonstrated the successful green crosslinking of salecan through its esterification with citric acid where the formation of strong covalent bonds collaboratively helped to stabilize the entire hydrogel systems in a wet state. Hydrogels presented a microporous morphology, good swelling capacity, pH responsiveness, great mechanical stability under stress conditions and good antibacterial activity, all related to the concentration of the biopolymers used in the synthesis step. Additionally, salecan hydrogels were preliminary investigated as printing inks. Thanks to their excellent rheological behavior, we optimized the citrate-salecan hydrogel inks and printing parameters to render 3D constructs with great printing fidelity and integrity. The novel synthesized salecan green crosslinked hydrogels enriches the family of salecan-derived hydrogels. Moreover, this work not only expands the application of salecan hydrogels in various fields, but also provides a new potential option of designing salecan-based 3D printed scaffolds for customized regenerative medicine.
可得然胶是一种多糖，由耐盐性农杆菌ZX09菌株产生。在本研究中，绿色交联柠檬酸-可得然胶水凝胶被探索为具有再生医学高应用潜力的新型材料。从整体物理化学性质和抗菌活性方面，深入研究并评估了可得然胶和柠檬酸对最终交联水凝胶的影响。傅里叶变换红外光谱（FTIR）表明，可得然胶通过与柠檬酸酯化成功实现了绿色交联，其中强共价键的形成共同有助于在湿态下稳定整个水凝胶体系。水凝胶呈现出微孔形态、良好的溶胀能力、pH响应性、在应力条件下具有良好的机械稳定性以及良好的抗菌活性，所有这些都与合成步骤中使用的生物聚合物浓度有关。此外，对可得然胶水凝胶作为印刷油墨进行了初步研究。由于其优异的流变行为，我们优化了柠檬酸盐-可得然胶水凝胶油墨和印刷参数，以获得具有高印刷保真度和完整性的3D构建体。新合成的可得然绿色交联水凝胶丰富了可得然衍生水凝胶家族。此外，这项工作不仅扩展了可得然胶水凝胶在各个领域的应用，还为定制再生医学设计基于可得然的3D打印支架提供了新的潜在选择。
