D'Addabbo Trifone, Ladurner Edith, Troccoli Alberto
Institute for Sustainable Plant Protection-CNR, 70126 Bari, BA, Italy.
CBC Europe-Biogard Division, 24050 Grassobbio, BG, Italy.
Plants (Basel). 2023 Feb 7;12(4):739. doi: 10.3390/plants12040739.
The nematicidal activity of garlic extracts is known on root-knot nematodes but never investigated on the grapevine nematode . In this study, the nematicidal activity of a commercial garlic extract formulate (GEF) was assessed on , both in vitro and in a pot assay. In the in vitro assays, mixed specimens of were exposed to a 0-4 mL L range of GEF concentrations, checking nematode immotility and mortality after 2, 4 or 8 h. In the experiments on potted grapevines, plants cultivated in soil infested by were irrigated twice at a 15-day interval with 0.05, 0.2 and 0.5 mL L solutions of GEF, including nontreated soil as a control. An almost complete mortality of specimens occurred after a 2 h exposure to a 2 mL L GEF concentration, while an 8 h exposure to even the 0.0312 and 0.0156 mL L solutions resulted in about 50% and 30% mortality, respectively. Soil treatment with a 0.5 mL L GEF solution significantly reduced the population of and increased the grapevine root growth compared to nontreated soil or soil treated with the lower dosages. Results of this study indicated that garlic-based nematicides could be an effective tool for management in organic and integrated vineyards.
大蒜提取物对线虫具有杀线虫活性，这在根结线虫方面是已知的，但从未在葡萄线虫上进行过研究。在本研究中，对一种商业大蒜提取物制剂（GEF）的杀线虫活性进行了体外和盆栽试验评估。在体外试验中，将混合的[线虫种类未明确]标本暴露于0 - 4 mL/L范围内的GEF浓度下，在2、4或8小时后检查线虫的不活动和死亡率。在盆栽葡萄的实验中，在受[线虫种类未明确]侵染的土壤中种植的植物，每隔15天用0.05、0.2和0.5 mL/L的GEF溶液灌溉两次，包括未处理的土壤作为对照。在暴露于2 mL/L的GEF浓度2小时后，[线虫种类未明确]标本几乎全部死亡，而即使暴露于0.0312和0.0156 mL/L的溶液8小时，死亡率分别约为50%和30%。与未处理的土壤或用较低剂量处理的土壤相比，用0.5 mL/L的GEF溶液进行土壤处理显著减少了[线虫种类未明确]的数量，并增加了葡萄根系的生长。本研究结果表明，基于大蒜的杀线虫剂可能是有机和综合葡萄园中线虫管理的有效工具。
