Huang Hong, Han Haohao, Lei Yayun, Qiao Huanhuan, Tang Daobin, Han Yonghui, Deng Zhenpeng, Mao Limin, Wu Xuli, Zhang Kai, Wang Jichun, Lv Changwen
College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China.
Key Laboratory of Biology and Genetic Breeding for Tuber and Root Crops in Chongqing, Beibei, Chongqing 400715, China.
Plants (Basel). 2023 Feb 20;12(4):957. doi: 10.3390/plants12040957.
Sweet potato virus disease (SPVD) is one of the main virus diseases in sweet potato [ (L.) Lam] that seriously affects the yield of sweet potato. Therefore, the establishment of a simple, rapid and effective method to detect SPVD is of great significance for the early warning and prevention of this disease. In this study, the experiment was carried out in two years to compare the grafting method and side grafting method for three sweet potato varieties, and the optimal grafting method was selected. After grafting with seedlings infected with SPVD, the symptomatic diagnosis and serological detection were performed in 86 host varieties, and the differences in SPVD resistance were determined by fluorescence quantitative PCR (qRT-PCR) and nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). The results showed that the survival rate of grafting by insertion method was significantly higher than that by side grafting method, and the disease resistance of different varieties to sweet potato virus disease was tested. The detection method established in this study can provide theoretical basis for identification and screening of resistant sweet potato varieties.
甘薯病毒病（SPVD）是甘薯[（L.）Lam]的主要病毒病之一，严重影响甘薯产量。因此，建立一种简单、快速、有效的SPVD检测方法对于该病的预警和防治具有重要意义。本研究通过两年试验，比较了三种甘薯品种的劈接法和侧接法，筛选出最佳嫁接方法。用感染SPVD的幼苗进行嫁接后，对86个寄主品种进行症状诊断和血清学检测，并通过荧光定量PCR（qRT-PCR）和硝酸纤维素膜酶联免疫吸附测定（NCM-ELISA）确定对SPVD抗性的差异。结果表明，劈接法嫁接成活率显著高于侧接法，且检测了不同品种对甘薯病毒病的抗病性。本研究建立的检测方法可为抗性甘薯品种的鉴定和筛选提供理论依据。
