Dong Yong-Hong, Fu Zhong, Zhang Ning-Nan, Shao Jing-Yi, Shen Jie, Yang En, Sun Shi-Yi, Zhao Zhi-Min, Xiao An, Liu Chen-Jian, Li Xiao-Ran
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.
Urology Department, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China.
Front Microbiol. 2023 Feb 10;14:1051437. doi: 10.3389/fmicb.2023.1051437. eCollection 2023.
Microbiota in the human body are closely related to human diseases. Female urogenital tract and rectal microbes have been considered as important factors affecting female pregnancy, but the mechanism is unknown.
Cervical, vaginal, urethral, and rectal swabs were collected from 22 infertile patients and 10 controls, and follicular fluid was extracted from 22 infertile patients. The microbial composition of different sampling sites of infertile patients was examined. By comparing the microbial composition difference between infertile patients and controls and combining bioinformatics methods to analyze the potential impact of the female urogenital tract (cervical, vaginal and urethral) and rectal microbial diversity on female infertility and pregnancy outcomes.
predominated in the female urogenital tract, but its abundance decreased in infertile patients, whereas the abundance of and increased. The microbial changes in the urethra had the same trend as that in the vagina. Compared with healthy controls, the cervical and rectal microbial diversity of infertile patients were significantly increased and decreased, respectively. There might be interactions between microbes in different parts of female. was enriched in the urogenital tract and rectum of infertile patients, and has a good predictive effect on infertility. Compared with infertile patients, was enriched in the vagina, urethra, and intestine of the control group. in follicular fluid might be associated with Non-pregnancy.
This study found that the microbial composition of infertile patients was changed compared with that of healthy people. The translocation of Lactobacillus between the rectum and urogenital tract might play a protective barrier role. The changes of and might be related to female infertility or pregnancy outcome. The study provided a theoretical basis for the future treatment of female infertility from the perspective of microorganisms by detecting the microbial changes associated with female infertility.
人体微生物群与人类疾病密切相关。女性泌尿生殖道和直肠微生物被认为是影响女性妊娠的重要因素，但其机制尚不清楚。
收集22例不孕患者和10例对照者的宫颈、阴道、尿道和直肠拭子，并从22例不孕患者中提取卵泡液。检测不孕患者不同采样部位的微生物组成。通过比较不孕患者与对照者的微生物组成差异，并结合生物信息学方法分析女性泌尿生殖道（宫颈、阴道和尿道）和直肠微生物多样性对女性不孕和妊娠结局的潜在影响。
在女性泌尿生殖道中占主导地位，但在不孕患者中其丰度降低，而 和 的丰度增加。尿道的微生物变化与阴道的趋势相同。与健康对照相比，不孕患者的宫颈和直肠微生物多样性分别显著增加和降低。女性不同部位的微生物之间可能存在相互作用。 在不孕患者的泌尿生殖道和直肠中富集，对不孕有较好的预测作用。与不孕患者相比， 在对照组的阴道、尿道和肠道中富集。卵泡液中的 可能与未妊娠有关。
本研究发现不孕患者的微生物组成与健康人相比发生了变化。直肠和泌尿生殖道之间的乳酸杆菌易位可能起到保护屏障作用。 和 的变化可能与女性不孕或妊娠结局有关。该研究通过检测与女性不孕相关的微生物变化，为未来从微生物角度治疗女性不孕提供了理论依据。
