Tomsia Marcin, Chełmecka Elżbieta, Głaz Małgorzata, Nowicka Joanna
Department of Forensic Medicine and Forensic Toxicology, Medical University of Silesia, 18 Medyków Street, 40-752 Katowice, Poland.
Department of Statistics, Department of Instrumental Analysis, Faculty of Pharmaceutical Sciences, Medical University of Silesia, Ostrogórska 30 Street, 41-200 Sosnowiec, Poland.
Toxics. 2023 Feb 5;11(2):152. doi: 10.3390/toxics11020152.
Alternative materials for postmortem diagnosis in the case of fatal poisonings are much needed when standard materials, such as blood and urine, are unavailable. The study presents a case of fatal mass methanol intoxication resulting from industrial alcohol consumption. The study aimed to determine methanol and formic acid concentrations in epiglottis cartilage, costal cartilage, and intervertebral disc cartilage and to analyze the correlation between their concentrations in cartilage tissues and the femoral blood. Methanol and formic acid concentrations in samples collected from 17 individuals ( = 17) were estimated using gas chromatography with flame ionization detection (GC-FID). Methanol concentration in the costal cartilage correlated with its concentration in the femoral blood (r = 0.871). Similar correlations were found for epiglottis cartilage (r = 0.822) and intervertebral disc cartilage (r = 0.892). Formic acid concentration in the blood correlated only with its concentration in urine (r = 0.784) and the epiglottis (r = 0.538). Cartilage tissue could serve as an alternative material for methanol analyses in postmortem studies. Formic acid, a methanol metabolite, does not meet the requirements for its presence determination in cartilage tissues.
在无法获取血液和尿液等标准材料的情况下，对于致命中毒病例，非常需要用于尸检诊断的替代材料。该研究报告了一例因饮用工业酒精导致的致命性大量甲醇中毒病例。该研究旨在测定会厌软骨、肋软骨和椎间盘软骨中的甲醇和甲酸浓度，并分析软骨组织中它们的浓度与股血中浓度之间的相关性。使用带有火焰离子化检测的气相色谱法（GC-FID）对从17名个体（n = 17）采集的样本中的甲醇和甲酸浓度进行了估算。肋软骨中的甲醇浓度与其在股血中的浓度相关（r = 0.871）。会厌软骨（r = 0.822）和椎间盘软骨（r = 0.892）也发现了类似的相关性。血液中的甲酸浓度仅与其在尿液中的浓度（r = 0.784）与会厌中的浓度（r = 0.538）相关。软骨组织可作为尸检研究中甲醇分析的替代材料。甲酸作为甲醇的代谢产物，不符合在软骨组织中测定其存在的要求。
