Kourelis Jiorgos, Marchal Clemence, Posbeyikian Andres, Harant Adeline, Kamoun Sophien
The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK.
Science. 2023 Mar 3;379(6635):934-939. doi: 10.1126/science.abn4116. Epub 2023 Mar 2.
Plant pathogens cause recurrent epidemics, threatening crop yield and global food security. Efforts to retool the plant immune system have been limited to modifying natural components and can be nullified by the emergence of new pathogen strains. Made-to-order synthetic plant immune receptors provide an opportunity to tailor resistance to pathogen genotypes present in the field. In this work, we show that plant nucleotide-binding, leucine-rich repeat immune receptors (NLRs) can be used as scaffolds for nanobody (single-domain antibody fragment) fusions that bind fluorescent proteins (FPs). These fusions trigger immune responses in the presence of the corresponding FP and confer resistance against plant viruses expressing FPs. Because nanobodies can be raised against most molecules, immune receptor-nanobody fusions have the potential to generate resistance against plant pathogens and pests delivering effectors inside host cells.
植物病原体引发反复出现的流行病，威胁着作物产量和全球粮食安全。对植物免疫系统进行改造的努力一直局限于修饰天然成分，并且可能因新病原体菌株的出现而失效。定制的合成植物免疫受体为针对田间存在的病原体基因型定制抗性提供了机会。在这项工作中，我们表明植物核苷酸结合、富含亮氨酸重复序列的免疫受体（NLRs）可作为与荧光蛋白（FPs）结合的纳米抗体（单域抗体片段）融合体的支架。这些融合体在相应的FP存在时触发免疫反应，并赋予对表达FPs的植物病毒的抗性。由于纳米抗体可以针对大多数分子产生，免疫受体 - 纳米抗体融合体有潜力产生针对在宿主细胞内递送效应子的植物病原体和害虫的抗性。
