Huang Zhangrao, Liu Gang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Bioeng Biotechnol. 2023 Feb 16;11:1039315. doi: 10.3389/fbioe.2023.1039315. eCollection 2023.
Prime editing (PE) is a precise genome manipulation technology based on the "search and replace" approach of the CRISPR-Cas9 system, while it does not require the exogenous donor DNA and the DNA double-strand breaks (DSBs). Comparing the base editing technology, the editing scope of prime editing has been widely expanded. Prime editing has been successfully applied in a variety of plant cells, animal cells and the model microorganism so far, and it has shown a good application potential in breeding and genomic functional study of animals and plants, disease treatment, and modification of the microbial strains. In this paper, the basic strategies of prime editing are briefly described, and its research progress is summarized and prospected from the application of multiple species. In addition, a variety of optimization strategies for improving its efficiency and specificity of prime editing are outlined.
碱基编辑（PE）是一种基于CRISPR-Cas9系统“搜索并替换”方法的精确基因组操作技术，同时它不需要外源供体DNA和DNA双链断裂（DSBs）。与碱基编辑技术相比，碱基编辑的编辑范围已得到广泛扩展。到目前为止，碱基编辑已成功应用于多种植物细胞、动物细胞和模式微生物中，并且在动植物育种和基因组功能研究、疾病治疗以及微生物菌株改造方面显示出良好的应用潜力。本文简要描述了碱基编辑的基本策略，并从多种物种的应用方面总结和展望了其研究进展。此外，还概述了提高碱基编辑效率和特异性的多种优化策略。
