Kang Yong Guk, Canoy Raymart Jay E, Jang Yongjun, Santos Ana Rita M P, Son Inwoo, Kim Beop-Min, Park Yongdoo
BK21 Four Institute of Precision Public Health, Korea University, Seoul 02841, Republic of Korea.
These authors contributed equally to this work.
Biomed Opt Express. 2023 Jan 4;14(2):577-592. doi: 10.1364/BOE.478264. eCollection 2023 Feb 1.
Biomedical researchers use optical coherence microscopy (OCM) for its high resolution in real-time label-free tomographic imaging. However, OCM lacks bioactivity-related functional contrast. We developed an OCM system that can measure changes in intracellular motility (indicating cellular process states) via pixel-wise calculations of intensity fluctuations from metabolic activity of intracellular components. To reduce image noise, the source spectrum is split into five using Gaussian windows with 50% of the full bandwidth. The technique verified that F-actin fiber inhibition by Y-27632 reduces intracellular motility. This finding could be used to search for other intracellular-motility-associated therapeutic strategies for cardiovascular diseases.
生物医学研究人员使用光学相干显微镜（OCM）进行实时无标记断层成像，因为其具有高分辨率。然而，OCM缺乏与生物活性相关的功能对比度。我们开发了一种OCM系统，该系统可以通过对细胞内成分代谢活动产生的强度波动进行逐像素计算，来测量细胞内运动性的变化（表明细胞过程状态）。为了减少图像噪声，使用具有50%全带宽的高斯窗口将源光谱分成五部分。该技术证实，Y-27632对F-肌动蛋白纤维的抑制作用会降低细胞内运动性。这一发现可用于寻找其他与细胞内运动性相关的心血管疾病治疗策略。
