Li Tian-Yao, Ye Chen, Zhang Yi-Jie, Zhang Jun-Xing, Yang Min, He Xia-Hong, Mei Xin-Yue, Liu Yi-Xiang, Zhu You-Yong, Huang Hui-Chuan, Zhu Shu-Sheng
School of Agriculture, Yunnan University, Kunming, 650500, China.
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Plant Divers. 2022 Mar 8;45(1):104-116. doi: 10.1016/j.pld.2022.02.003. eCollection 2023 Jan.
Compared with the use of monocultures in the field, cultivation of medicinal herbs in forests is an effective strategy to alleviate disease. Chemical interactions between herbs and trees play an important role in disease suppression in forests. We evaluated the ability of leachates from needles of to induce resistance in leaves, identified the components via gas chromatography-mass spectrometry (GC-MS), and then deciphered the mechanism of 2,3-Butanediol as the main component in the leachates responsible for resistance induction via RNA sequencing (RNA-seq). Prespraying leachates and 2,3-Butanediol onto leaves could induce the resistance of to . The RNA-seq results showed that prespraying 2,3-Butanediol onto leaves with or without infection upregulated the expression of large number of genes, many of which are involved in transcription factor activity and the mitogen-activated protein kinase (MAPK) signaling pathway. Specifically, 2,3-Butanediol spraying resulted in jasmonic acid (JA) -mediated induced systemic resistance (ISR) by activating MYC2 and ERF1. Moreover, 2,3-Butanediol induced systemic acquired resistance (SAR) by upregulating pattern-triggered immunity (PTI)- and effector-triggered immunity (ETI)-related genes and activated camalexin biosynthesis through activation of WRKY33. Overall, 2,3-Butanediol from the leachates of pine needles could activate the resistance of to leaf disease infection through ISR, SAR and camalexin biosynthesis. Thus, 2,3-Butanediol is worth developing as a chemical inducer for agricultural production.
与在田间使用单一栽培相比，在森林中种植药用植物是减轻病害的一种有效策略。草药与树木之间的化学相互作用在森林病害抑制中起着重要作用。我们评估了松针浸出液诱导植物叶片产生抗性的能力，通过气相色谱-质谱联用仪（GC-MS）鉴定了其成分，然后通过RNA测序（RNA-seq）解析了浸出液中作为主要成分的2,3-丁二醇诱导抗性的机制。在叶片上预喷浸出液和2,3-丁二醇可以诱导植物对病害的抗性。RNA-seq结果表明，在有或没有病害感染的情况下，在叶片上预喷2,3-丁二醇会上调大量基因的表达，其中许多基因参与转录因子活性和丝裂原活化蛋白激酶（MAPK）信号通路。具体而言，喷洒2,3-丁二醇通过激活MYC2和ERF1导致茉莉酸（JA）介导的诱导系统抗性（ISR）。此外，2,3-丁二醇通过上调模式触发免疫（PTI）和效应子触发免疫（ETI）相关基因诱导系统获得性抗性（SAR），并通过激活WRKY33激活植保素生物合成。总体而言，松针浸出液中的2,3-丁二醇可以通过ISR、SAR和植保素生物合成激活植物对叶部病害感染的抗性。因此，2,3-丁二醇作为一种农业生产中的化学诱导剂值得开发。
