Zdraljevic Stefan, Walter-McNeill Laura, Marquez Heriberto, Kruglyak Leonid
Department of Human Genetics, University of California, Los Angeles, CA, USA.
Department of Biological Chemistry, University of California, Los Angeles, CA, USA.
MicroPubl Biol. 2023 Feb 17;2023. doi: 10.17912/micropub.biology.000775. eCollection 2023.
Identification of the genetic basis of phenotypic variation within species remains challenging. In species with low recombination rates, such as , genomic regions linked to a phenotype of interest by genetic mapping studies are often large, making it difficult to identify the specific genes and DNA sequence variants that underlie phenotypic differences. Here, we introduce a method that enables researchers to induce heritable targeted recombination in with Cas9. We demonstrate that high rates of targeted nonhomologous recombination can be induced by Cas9 in a genomic region in which naturally occurring meiotic recombination events are exceedingly rare. We anticipate that Cas9-induced nonhomologous recombination (CINR) will greatly facilitate high-resolution genetic mapping in this species.
确定物种内表型变异的遗传基础仍然具有挑战性。在重组率较低的物种中，例如，通过遗传图谱研究与感兴趣的表型相关的基因组区域通常很大，这使得难以确定构成表型差异基础的特定基因和DNA序列变异。在这里，我们介绍了一种方法，使研究人员能够在中用Cas9诱导可遗传的靶向重组。我们证明，在自然发生的减数分裂重组事件极为罕见的基因组区域中，Cas9可以诱导高频率的靶向非同源重组。我们预计，Cas9诱导的非同源重组（CINR）将极大地促进该物种的高分辨率遗传图谱绘制。
