Haxim Yakupjan, Wang Lei, Pan Zhendong, Fan Xiaorong, Ma Jinbiao
State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, China.
Xinjiang Key Laboratory of Conservation and Utilization of Plant Gene Resources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, China.
Front Plant Sci. 2023 Feb 20;14:1104070. doi: 10.3389/fpls.2023.1104070. eCollection 2023.
High-affinity K transporters (HKTs) are known as transmembrane cation transporters and are involved in Na or Na-K transport in plants. In this study, a novel HKT gene was isolated and characterized from the halophyte, . It belongs to subfamily I of HKT and shows high homology with other halophyte HKT proteins. Functional characterization of indicated that it contributes to facilitating Na uptake in Na-sensitive yeast strains G19, however, cannot rescue the K uptake-defective phenotype of yeast strain CY162, demonstrating SeHKT1;2 selectively transports Na rather than K. The addition of K along with NaCl relieved the Na sensitivity. Furthermore, heterologous expression of in mutant of increased salt sensitivity and could not rescued the transgenic plants. This study will provide valuable gene resources for improving the salt tolerance in other crops by genetic engineering.
高亲和性钾转运体（HKTs）是已知的跨膜阳离子转运体，参与植物中的钠或钠钾转运。在本研究中，从盐生植物中分离并鉴定了一个新的HKT基因。它属于HKT亚家族I，与其他盐生植物HKT蛋白具有高度同源性。对其功能特性的研究表明，它有助于促进钠敏感酵母菌株G19对钠的吸收，然而，不能挽救酵母菌株CY162的钾吸收缺陷表型，表明SeHKT1;2选择性转运钠而非钾。同时添加钾和氯化钠可缓解钠敏感性。此外，在突变体中异源表达会增加盐敏感性，且不能挽救转基因植物。本研究将为通过基因工程提高其他作物的耐盐性提供有价值的基因资源。
