Hyeon Ji-Yeon, Tseren-Ochir Erdene-Ochir, Lee Dong-Hun, Nahm Sang-Soep, Gladue Douglas P, Borca Manuel V, Song Chang-Seon, Risatti Guillermo R
Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT, United States.
College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
Front Vet Sci. 2023 Feb 20;10:1094052. doi: 10.3389/fvets.2023.1094052. eCollection 2023.
African swine fever (ASF) is a highly contagious and fatal disease affecting domestic and wild pigs caused by the African swine fever virus (ASFV). Since the first outbreak in China in August 2018, ASF has spread rapidly in Asia. and the first case in Mongolia was confirmed in January 2019. In this study, we report the first whole genome sequence of an ASFV (ASFV SS-3/Mongolia/2019) detected from a backyard pig in Mongolia in February 2019 using whole genome sequencing. We analyzed their phylogenetic relationship with other genotype II ASFVs from Eurasia. The ASFV SS-3/Mongolia/2019 belonged to genotype II (p72 and p54), serogroup 8 (CD2v), Tet-10a variant (pB602L), and IGRIII variant (intergenic region between the I73R/I329L genes). A total of five amino acid substitutions were observed in MGF 360-10L, MGF 505-4R, MGF 505-9R, NP419L, and I267L genes compared to the ASFV Georgia 2007/1 virus. ML phylogenetic analysis of the whole genome sequence showed that the virus shares a high nucleotide sequence identity with ASFVs recently identified in Eastern Europe and Asia and clustered with the ASFV/Zabaykali/WB5314/2020|Russia|2020 virus which was identified at the border between the Russian Federation and Mongolia in 2020. Our results suggest that trans boundary spread of ASF occurred through close geographic proximity.
非洲猪瘟（ASF）是一种由非洲猪瘟病毒（ASFV）引起的、极具传染性且致命的疾病，可感染家猪和野猪。自2018年8月在中国首次爆发以来，ASF在亚洲迅速蔓延。2019年1月，蒙古确认了首例病例。在本研究中，我们报告了2019年2月从蒙古一头后院猪中检测到的一株ASFV（ASFV SS-3/蒙古/2019）的首个全基因组序列，该序列通过全基因组测序获得。我们分析了它与来自欧亚大陆的其他II型ASFV的系统发育关系。ASFV SS-3/蒙古/2019属于II型（p72和p54）、血清群8（CD2v）、Tet-10a变体（pB602L）和IGRIII变体（I73R/I329L基因之间的基因间隔区）。与ASFV格鲁吉亚2007/1病毒相比，在MGF 360-10L、MGF 505-4R、MGF 505-9R、NP419L和I267L基因中总共观察到五个氨基酸替换。全基因组序列的ML系统发育分析表明，该病毒与最近在东欧和亚洲鉴定出的ASFV具有高度的核苷酸序列同一性，并与2020年在俄罗斯联邦和蒙古边境鉴定出的ASFV/ Zabaykali/WB5314/2020|俄罗斯|2020病毒聚类。我们的结果表明，ASF的跨界传播是通过地理位置接近而发生的。
