Sun Jing, Bie Xiao Min, Chu Xiao Li, Wang Ning, Zhang Xian Sheng, Gao Xin-Qi
National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
Front Plant Sci. 2023 Feb 21;14:1142779. doi: 10.3389/fpls.2023.1142779. eCollection 2023.
Tillering is a critical agronomic trait of wheat ( L.) that determines the shoot architecture and affects grain yield. (), encoding a phosphatidylethanolamine-binding protein, is implicated in the transition to flowering and shoot architecture in plant development. However, the roles of TFL1 homologs is little known in wheat development. CRISPR/Cas9-mediated targeted mutagenesis was used in this study to generate a set of wheat (Fielder) mutants with single, double or triple-null alleles. The wheat mutations decreased the tiller number per plant in the vegetative growth stage and the effective tiller number per plant and spikelet number per spike at maturity in the field. RNA-seq analysis showed that the expression of the auxin signaling-related and cytokinin signaling-related genes was significantly changed in the axillary buds of mutant seedlings. The results suggested that wheat were implicated in tiller regulation by auxin and cytokinin signaling.
分蘖是小麦的一个关键农艺性状，它决定了茎蘖结构并影响谷物产量。（）编码一种磷脂酰乙醇胺结合蛋白，参与植物发育过程中向开花和茎蘖结构的转变。然而，TFL1同源物在小麦发育中的作用鲜为人知。本研究利用CRISPR/Cas9介导的靶向诱变技术，产生了一系列具有单、双或三无效等位基因的小麦（费ielder）突变体。小麦突变体在营养生长阶段降低了单株分蘖数，在田间成熟时降低了单株有效分蘖数和每穗小穗数。RNA测序分析表明，生长素信号相关基因和细胞分裂素信号相关基因在突变体幼苗腋芽中的表达发生了显著变化。结果表明，小麦通过生长素和细胞分裂素信号参与分蘖调控。
